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Cathepsin S of Sciaenops ocellatus: Identification, transcriptional expression and enzymatic activity
Sun, Bo-guang1,2; Chi, Heng1,2
2016
发表期刊INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷号82期号:2016页码:76-82
文章类型Article
摘要Cathepsin S is a member of cysteine cathepsins and belongs to the cathepsin L-like family. In mammals, it is known to participate in various physiological processes and host immune defense. In teleost fish, the function of cathepsin S is less investigated. In the present work, we characterized a cathepsin S homologue (SoCatS) from red drum (Sciaenops ocellatus), a commercially valuable fish in Chinese mariculture. Like all cathepsin S, SoCatS possesses a peptidase domain with four catalytically essential residues (Gln140, Cys146, His285, and Asn305) conserved in the cathepsin S of different organisms. SoCatS shares 60-90% overall sequence identities with known teleost cathepsin S. Phylogenetic profiling indicated that SoCatS is evolutionally close to the cathepsin S of other teleost fish, especially Miichthys miiuy, a member of Sciaenidae family like red drum. SoCatS expression was detected in various tissues and was enhanced by bacterial infection. Purified recombinant SoCatS exhibited apparent peptidase activity with maximum at 50 degrees C and pH 7.5. This activity depended on the catalytic residue Cys146 and was severely reduced by the cathepsin inhibitor E-64. Our results suggest that SoCatS functions as a cysteine protease which is probably involved in the antibacterial immunity of red drum. (C) 2015 Elsevier B.V. All rights reserved.
关键词Cathepsin s Sciaenops Ocellatus Teleost Fish
DOI10.1016/j.ijbiomac.2015.10.037
收录类别SCI
语种英语
WOS记录号WOS:000367425100011
引用统计
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/86650
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
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Sun, Bo-guang,Chi, Heng. Cathepsin S of Sciaenops ocellatus: Identification, transcriptional expression and enzymatic activity[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2016,82(2016):76-82.
APA Sun, Bo-guang,&Chi, Heng.(2016).Cathepsin S of Sciaenops ocellatus: Identification, transcriptional expression and enzymatic activity.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,82(2016),76-82.
MLA Sun, Bo-guang,et al."Cathepsin S of Sciaenops ocellatus: Identification, transcriptional expression and enzymatic activity".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 82.2016(2016):76-82.
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