| Purification and biochemical characterization of an alkaline protease from marine bacteria Pseudoalteromonas sp 129-1 | |
| Wu, Shimei1; Liu, Ge2; Zhang, Dechao3; Li, Chaoxu1; Sun, Chaomin2 | |
| 2015-12-01 | |
| 发表期刊 | JOURNAL OF BASIC MICROBIOLOGY
 |
| 卷号 | 55期号:12页码:1427-1434 |
| 文章类型 | Article |
| 摘要 | An extracellular alkaline protease produced by marine bacteria strain Pseudoalteromonas sp. 129-1 was purified by ammonium sulphate precipitation, anion exchange chromatography, and gel filtration. The purity of the protease was confirmed by SDS-PAGE and molecular mass was estimated to be 35kDa. The protease maintained considerable activity and stability at a wide temperature range of 10-60 degrees C and pH range of 6-11, and optimum activity was detected at temperature of 50 degrees C and pH of 8. Metallo-protease inhibitor, EDTA, had no inhibitory effect on protease activity even at concentration up to 15mM, whereas 15mM PMSF, a common serine protease inhibitor, greatly inactivated the protease. The high stability of the protease in the presence of surfactants (SDS, Tween 80, and Triton X-100), oxidizing agent H2O2, and commercial detergents was observed. Moreover, the protease was tolerant to most of the tested organic solvents, and saline tolerant up to 30%. Interestingly, biofilm of Pseudomonas aeruginosa PAO1 was greatly reduced by 0.01mgml(-1) of the protease, and nearly completely abolished with the concentration of 1mgml(-1). Collectively, the protease showed valuable feathers as an additive in laundry detergent and non-toxic anti-biofilm agent. |
| 关键词 | Pseudoalteromonas Alkaline Protease Purification Surfactant Biofilm |
| DOI | 10.1002/jobm.201500327 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000366143300009 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/86614 |
| 专题 | 海洋生物分类与系统演化实验室 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biobased Mat, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Shimei,Liu, Ge,Zhang, Dechao,et al. Purification and biochemical characterization of an alkaline protease from marine bacteria Pseudoalteromonas sp 129-1[J]. JOURNAL OF BASIC MICROBIOLOGY,2015,55(12):1427-1434. |
| APA | Wu, Shimei,Liu, Ge,Zhang, Dechao,Li, Chaoxu,&Sun, Chaomin.(2015).Purification and biochemical characterization of an alkaline protease from marine bacteria Pseudoalteromonas sp 129-1.JOURNAL OF BASIC MICROBIOLOGY,55(12),1427-1434. |
| MLA | Wu, Shimei,et al."Purification and biochemical characterization of an alkaline protease from marine bacteria Pseudoalteromonas sp 129-1".JOURNAL OF BASIC MICROBIOLOGY 55.12(2015):1427-1434. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Purification and bio（434KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论