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Purification and biochemical characterization of an alkaline protease from marine bacteria Pseudoalteromonas sp 129-1
Wu, Shimei1; Liu, Ge2; Zhang, Dechao3; Li, Chaoxu1; Sun, Chaomin2
2015-12-01
Source PublicationJOURNAL OF BASIC MICROBIOLOGY
Volume55Issue:12Pages:1427-1434
SubtypeArticle
AbstractAn extracellular alkaline protease produced by marine bacteria strain Pseudoalteromonas sp. 129-1 was purified by ammonium sulphate precipitation, anion exchange chromatography, and gel filtration. The purity of the protease was confirmed by SDS-PAGE and molecular mass was estimated to be 35kDa. The protease maintained considerable activity and stability at a wide temperature range of 10-60 degrees C and pH range of 6-11, and optimum activity was detected at temperature of 50 degrees C and pH of 8. Metallo-protease inhibitor, EDTA, had no inhibitory effect on protease activity even at concentration up to 15mM, whereas 15mM PMSF, a common serine protease inhibitor, greatly inactivated the protease. The high stability of the protease in the presence of surfactants (SDS, Tween 80, and Triton X-100), oxidizing agent H2O2, and commercial detergents was observed. Moreover, the protease was tolerant to most of the tested organic solvents, and saline tolerant up to 30%. Interestingly, biofilm of Pseudomonas aeruginosa PAO1 was greatly reduced by 0.01mgml(-1) of the protease, and nearly completely abolished with the concentration of 1mgml(-1). Collectively, the protease showed valuable feathers as an additive in laundry detergent and non-toxic anti-biofilm agent.
KeywordPseudoalteromonas Alkaline Protease Purification Surfactant Biofilm
DOI10.1002/jobm.201500327
Indexed BySCI
Language英语
WOS IDWOS:000366143300009
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/86614
Collection海洋生物分类与系统演化实验室
Affiliation1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biobased Mat, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
Recommended Citation
GB/T 7714
Wu, Shimei,Liu, Ge,Zhang, Dechao,et al. Purification and biochemical characterization of an alkaline protease from marine bacteria Pseudoalteromonas sp 129-1[J]. JOURNAL OF BASIC MICROBIOLOGY,2015,55(12):1427-1434.
APA Wu, Shimei,Liu, Ge,Zhang, Dechao,Li, Chaoxu,&Sun, Chaomin.(2015).Purification and biochemical characterization of an alkaline protease from marine bacteria Pseudoalteromonas sp 129-1.JOURNAL OF BASIC MICROBIOLOGY,55(12),1427-1434.
MLA Wu, Shimei,et al."Purification and biochemical characterization of an alkaline protease from marine bacteria Pseudoalteromonas sp 129-1".JOURNAL OF BASIC MICROBIOLOGY 55.12(2015):1427-1434.
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