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The Roles of Two miRNAs in Regulating the Immune Response of Sea Cucumber
Zhang, Pengjuan1; Li, Chenghua1; Zhang, Ran1; Zhang, Weiwei1; Jin, Chunhua1; Wang, Lingling2; Song, Linsheng2
2015-12-01
Source PublicationGENETICS
Volume201Issue:4Pages:1397-+
SubtypeArticle
AbstractMicroRNAs (miRNAs) have emerged as key regulators in many pathological processes by suppressing the transcriptional and post-transcriptional expression of target genes. MiR-2008 was previously found to be significantly up-regulated in diseased sea cucumber Apostichopus japonicus by high-through sequencing, whereas the reads of miR-137, a well-documented tumor repressor, displayed no significant change. In the present study, we found that miR-137 expression was slightly attenuated and miR-2008 was significantly enhanced after Vibrio splendidus infection or Lipopolysaccharides application. Further target screening and dual-luciferase reporter assay revealed that the two important miRNAs shared a common target gene of betaine-homocysteine S-methyltransferase (AjBHMT), which exhibited noncorrelated messenger RNA and protein expression patterns after bacterial challenge. In order to fully understand their regulatory mechanisms, we conducted the functional experiments in vitro and in vivo. The overexpression of miR-137 in sea cucumber or primary coelomocytes significantly decreased, whereas the inhibition of miR-137 increased the mRNA and protein expression levels of AjBHMT. In contrast, miR-2008 overexpression and inhibition showed no effect on AjBHMT mRNA levels, but the concentration of AjBHMT protein displayed significant changes both in vitro and in vivo. Consistently, the homocysteine (Hcy) contents were also accordingly altered in the aberrant expression analysis of both miRNAs, consistent with the results of the AjBHMT silencing assay in vitro and in vivo. More importantly, small interfering RNA mediated AjBHMT knockdown and Hcy exposure analyses both significantly increased reactive oxygen species (ROS) production and decreased the number of surviving invasive pathogen in sea cucumber coelomocytes. Taken together, these findings confirmed the differential roles of sea cucumber miR-137 and miR-2008 in regulating the common target AjBHMT to promote ROS production and the clearance of pathogenic microorganisms through Hcy accumulation.
KeywordApostichopus Japonicus Mir-137 Mir-2008 Betaine-homocysteine S-methyltransferase Homocysteine Ros Genetics Of Immunity
DOI10.1534/genetics.115.178871
Indexed BySCI
Language英语
WOS IDWOS:000366386500010
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/86605
Collection实验海洋生物学重点实验室
Affiliation1.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China
2.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Pengjuan,Li, Chenghua,Zhang, Ran,et al. The Roles of Two miRNAs in Regulating the Immune Response of Sea Cucumber[J]. GENETICS,2015,201(4):1397-+.
APA Zhang, Pengjuan.,Li, Chenghua.,Zhang, Ran.,Zhang, Weiwei.,Jin, Chunhua.,...&Song, Linsheng.(2015).The Roles of Two miRNAs in Regulating the Immune Response of Sea Cucumber.GENETICS,201(4),1397-+.
MLA Zhang, Pengjuan,et al."The Roles of Two miRNAs in Regulating the Immune Response of Sea Cucumber".GENETICS 201.4(2015):1397-+.
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