Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Characterization of a Newly Isolated Marine Fungus Aspergillus dimorphicus for Optimized Production of the Anti-Tumor Agent Wentilactones | |
Xu, Rui1,2 ; Xu, Gang-Ming1; Li, Xiao-Ming1; Li, Chun-Shun1; Wang, Bin-Gui1
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| 2015-11-01 | |
| 发表期刊 | MARINE DRUGS
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| 卷号 | 13期号:11页码:7040-7054 |
| 文章类型 | Article |
| 摘要 | The potential anti-tumor agent wentilactones were produced by a newly isolated marine fungus Aspergillus dimorphicus. This fungus was derived from deep-sea sediment and identified by polyphasic approach, combining phenotypic, molecular, and extrolite profiles. However, wentilactone production was detected only under static cultures with very low yields. In order to improve wentilactone production, culture conditions were optimized using the response surface methodology. Under the optimal static fermentation conditions, the experimental values were closely consistent with the prediction model. The yields of wentilactone A and B were increased about 11-fold to 13.4 and 6.5 mg/L, respectively. The result was further verified by fermentation scale-up for wentilactone production. Moreover, some small-molecule elicitors were found to have capacity of stimulating wentilactone production. To our knowledge, this is first report of optimized production of tetranorlabdane diterpenoids by a deep-sea derived marine fungus. The present study might be valuable for efficient production of wentilactones and fundamental investigation of the anti-tumor mechanism of norditerpenoids. |
| 关键词 | Aspergillus Dimorphicus Anti-tumor Agent Wentilactones Optimization |
| DOI | 10.3390/md13117040 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000365645400025 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/86533 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Xu, Rui,Xu, Gang-Ming,Li, Xiao-Ming,et al. Characterization of a Newly Isolated Marine Fungus Aspergillus dimorphicus for Optimized Production of the Anti-Tumor Agent Wentilactones[J]. MARINE DRUGS,2015,13(11):7040-7054. |
| APA | Xu, Rui,Xu, Gang-Ming,Li, Xiao-Ming,Li, Chun-Shun,&Wang, Bin-Gui.(2015).Characterization of a Newly Isolated Marine Fungus Aspergillus dimorphicus for Optimized Production of the Anti-Tumor Agent Wentilactones.MARINE DRUGS,13(11),7040-7054. |
| MLA | Xu, Rui,et al."Characterization of a Newly Isolated Marine Fungus Aspergillus dimorphicus for Optimized Production of the Anti-Tumor Agent Wentilactones".MARINE DRUGS 13.11(2015):7040-7054. |
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| Characterization of （1737KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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