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Metabolic engineering of Escherichia coli to improve recombinant protein production
Liu, Min1,2; Feng, Xinjun1; Ding, Yamei3; Zhao, Guang1; Liu, Huizhou1; Xian, Mo1
2015-12-01
Source PublicationAPPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume99Issue:24Pages:10367-10377
SubtypeReview
AbstractEscherichia coli is one of the most widely used strains for recombinant protein production. However, obstacles also exist in both academic researches and industrial applications, such as the metabolic burden, the carbon source waste, and the cells' physiological deterioration. This article reviews recent approaches for improving recombinant protein production in metabolic engineering, including workhorse selection, stress factor application, and carbon flux regulation. Selecting a suitable host is the first key point for recombinant protein production. In general, it all depends on characteristics of the strains and the target proteins. It will be triggered cells physiological deterioration when the medium is significantly different from the cell's natural environment. Coexpression of stress factors can help proteins to fold into their native conformation. Carbon flux regulation is a direct approach for redirecting more carbon flux toward the desirable pathways and products. However, some undesirable consequences are usually found in metabolic engineering, such as glucose transport inhibition, cell growth retardation, and useless metabolite accumulation. More efficient regulators and platform cell factories should be explored to meet a variety of production demands.
KeywordRecombinant Protein Production Metabolic Engineering Workhorse Selection Stress Factors Carbon Flux Regulation Growth Retardation
DOI10.1007/s00253-015-6955-9
Indexed BySCI
Language英语
WOS IDWOS:000365712300001
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/86531
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Key Lab Biobased Mat, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
Recommended Citation
GB/T 7714
Liu, Min,Feng, Xinjun,Ding, Yamei,et al. Metabolic engineering of Escherichia coli to improve recombinant protein production[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2015,99(24):10367-10377.
APA Liu, Min,Feng, Xinjun,Ding, Yamei,Zhao, Guang,Liu, Huizhou,&Xian, Mo.(2015).Metabolic engineering of Escherichia coli to improve recombinant protein production.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,99(24),10367-10377.
MLA Liu, Min,et al."Metabolic engineering of Escherichia coli to improve recombinant protein production".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 99.24(2015):10367-10377.
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