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TLR7 is required for optimal immune defense against bacterial infection in tongue sole (Cynoglossus semilaevis)
Li, Xue-Peng1,2,3; Sun, Li1,3
2015-11-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume47Issue:1Pages:93-99
SubtypeArticle
AbstractIn mammals as well as in teleost, toll-like receptor 7 (TLR7) is known to be involved in antiviral immunity by recognizing viral RNA. However, the antibacterial potential of fish TLR7 is unclear. In this study, we analyzed the TLR7 of tongue sole (Cynoglossus semilaevis), CsTLR7, and examined its potential involvement in antibacterial immunity. CsTLR7 is composed of 1052 amino acid residues and shares 64.0%-75.9% overall sequence identities with known teleost TLR7. CsTLR7 possesses a toll/interleukin-1 receptor domain and six leucine-rich repeats. Constitutive expression of CsTLR7 occurred in relatively high levels in kidney, spleen and liver. Bacterial infection upregulated CsTLR7 expression, whereas viral infection downregulated CsTLR7 expression. Knockdown of CsTLR7 significantly enhanced bacterial dissemination in the tissues of tongue sole. Treatment of tongue sole with the imidazoquinoline compound R848 (TLR7 activator) and the endosomal acidification inhibitor chloroquine (TLR7 inhibitor) caused enhanced and reduced resistance against bacterial infection respectively. These results indicate that CsTLR7 plays an essential role in the antibacterial immunity of tongue sole. (C) 2015 Elsevier Ltd. All rights reserved.
KeywordToll-like Receptor Cynoglossus Semilaevis R848 Chloroquine Innate Immunity Antibacterial
DOI10.1016/j.fsi.2015.08.025
Indexed BySCI
Language英语
WOS IDWOS:000365053300011
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/82320
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Qingdao Natl Oceanog Lab, Funct Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Li, Xue-Peng,Sun, Li. TLR7 is required for optimal immune defense against bacterial infection in tongue sole (Cynoglossus semilaevis)[J]. FISH & SHELLFISH IMMUNOLOGY,2015,47(1):93-99.
APA Li, Xue-Peng,&Sun, Li.(2015).TLR7 is required for optimal immune defense against bacterial infection in tongue sole (Cynoglossus semilaevis).FISH & SHELLFISH IMMUNOLOGY,47(1),93-99.
MLA Li, Xue-Peng,et al."TLR7 is required for optimal immune defense against bacterial infection in tongue sole (Cynoglossus semilaevis)".FISH & SHELLFISH IMMUNOLOGY 47.1(2015):93-99.
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