IOCAS-IR  > 实验海洋生物学重点实验室
An LRR-only protein representing a new type of pattern recognition receptor in Chlamys farreri
Wang, Mengqiang1; Wang, Lingling1,3; Guo, Ying1; Yi, Qilin2; Song, Linsheng2
2016
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume54Issue:1Pages:145-155
SubtypeArticle
AbstractAccumulating evidence has demonstrated that leucine-rich repeat (LRR)-only proteins could mediate protein-ligand and protein protein interactions and were involved in the immune response. In the present study, an LRR-only protein (designed as CfLRRop-1) was cloned from Zhikong scallop Chlamys farreri. The complete cDNA sequence of CfLRRop-1 contained an open reading frame (ORF) of 1377 bp, which encoded a protein of 458 amino acids. An LRRNT motif, an LRR_7 motif and seven LRR motifs were found in the deduced amino acid sequence of CfLRRop-1. And these seven LRR motifs contained a conserved signature sequence LxxLxxNxL. The mRNA transcripts of CfLRRop-1 were constitutively expressed in all the tested tissues, including haemocytes, muscle, mantle, gill, hepatopancreas and gonad, with the highest expression level in hepatopancreas. After the stimulation of lipopolysaccharide (LPS), peptidoglycan (PGN), glucan (GLU) and polyinosinic-polycytidylic acid (poly I:C), the mRNA transcripts of CARRop-1 in haemocytes all increased firstly within the first 6 h and secondly during 12-24 h post stimulation. The mRNA expression level of CfLRRop-1 was continuously up-regulated, after the expression of CfTLR (previously identified Toll-like receptor in C. farreri) was suppressed via RNA interference (RNAi). The recombinant CfLRRop-1 protein could directly bind LPS, PGN, GLU and poly I:C, and induce the release of TNF-alpha in mixed primary cultured scallop haemocytes. These results collectively indicated that CfLRRop-1 would function as a powerful pattern recognition receptor (PRR) and play a pivotal role in the immune response of scallops. (C) 2015 Elsevier Ltd. All rights reserved.
KeywordChlamys Farreri Innate Immunity Leucine-rich Repeat Lrr-only Protein Pattern Recognition Receptor
DOI10.1016/j.dci.2015.09.006
Indexed BySCI
Language英语
WOS IDWOS:000364883100015
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/82299
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Dalian Ocean Univ, Minist Agr, Key Lab Mariculture & Stock Enhancement North Chi, Dalian 116023, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
First Author Affilication中国科学院海洋研究所
Recommended Citation
GB/T 7714
Wang, Mengqiang,Wang, Lingling,Guo, Ying,et al. An LRR-only protein representing a new type of pattern recognition receptor in Chlamys farreri[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2016,54(1):145-155.
APA Wang, Mengqiang,Wang, Lingling,Guo, Ying,Yi, Qilin,&Song, Linsheng.(2016).An LRR-only protein representing a new type of pattern recognition receptor in Chlamys farreri.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,54(1),145-155.
MLA Wang, Mengqiang,et al."An LRR-only protein representing a new type of pattern recognition receptor in Chlamys farreri".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 54.1(2016):145-155.
Files in This Item:
File Name/Size DocType Version Access License
An LRR-only protein (2327KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, Mengqiang]'s Articles
[Wang, Lingling]'s Articles
[Guo, Ying]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, Mengqiang]'s Articles
[Wang, Lingling]'s Articles
[Guo, Ying]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, Mengqiang]'s Articles
[Wang, Lingling]'s Articles
[Guo, Ying]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: An LRR-only protein representing a new type of pattern recognition receptor in Chlamys farreri.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.