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Isolation, expression and characterization of rbcL gene from Ulva prolifera J. Agardh (Ulvophyceae, Chlorophyta)
Shao Zhanru1; Li Wei1,2; Guo Hui1,3; Duan Delin1
2015-12-01
Source PublicationJOURNAL OF OCEAN UNIVERSITY OF CHINA
Volume14Issue:6Pages:1087-1095
SubtypeArticle
AbstractUlva prolifera is a typical green alga in subtidal areas and can grow tremendously fast. A highly efficient Rubisco enzyme which is encoded by UpRbcL gene may contribute to the rapid growth. In this study, the full-length UpRbcL open reading frame (ORF) was identified, which encoded a protein of 474 amino acids. Phylogenetic analysis of UpRbcL sequences revealed that Chlorophyta had a closer genetic relationship with higher plants than with Rhodophyta and Phaeophyta. The two distinct residues (aa11 and aa91) were presumed to be unique for Rubisco catalytic activity. The predicted three-dimensional structure showed that one alpha/beta-barrel existed in the C-terminal region, and the sites for Mg2+ coordination and CO2 fixation were also located in this region. Gene expression profile indicated that UpRbcL was expressed at a higher level under light exposure than in darkness. When the culture temperature reached 35A degrees C, the expression level of UpRbcL was 2.5-fold lower than at 15A degrees C, and the carboxylase activity exhibited 13.8-fold decrease. UpRbcL was heterologously expressed in E. coli and was purified by Ni2+ affinity chromatography. The physiological and biochemical characterization of recombinant Rubisco will be explored in the future.
KeywordRibulose-1 5-bisphosphate Carboxylase/oxygenase Large Subunit Sequence Analysis Real-time Pcr In Vitro Expression Ulva Prolifera
DOI10.1007/s11802-015-2661-6
Indexed BySCI
Language英语
WOS IDWOS:000364572000021
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Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/82291
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Univ Jena, Inst Gen Bot & Plant Physiol, D-07743 Jena, Germany
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Shao Zhanru,Li Wei,Guo Hui,et al. Isolation, expression and characterization of rbcL gene from Ulva prolifera J. Agardh (Ulvophyceae, Chlorophyta)[J]. JOURNAL OF OCEAN UNIVERSITY OF CHINA,2015,14(6):1087-1095.
APA Shao Zhanru,Li Wei,Guo Hui,&Duan Delin.(2015).Isolation, expression and characterization of rbcL gene from Ulva prolifera J. Agardh (Ulvophyceae, Chlorophyta).JOURNAL OF OCEAN UNIVERSITY OF CHINA,14(6),1087-1095.
MLA Shao Zhanru,et al."Isolation, expression and characterization of rbcL gene from Ulva prolifera J. Agardh (Ulvophyceae, Chlorophyta)".JOURNAL OF OCEAN UNIVERSITY OF CHINA 14.6(2015):1087-1095.
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