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Determination of Reduced Nicotinamide Adenine Dinucleotide with a Protamine Multiwalled Carbon Nanotube Electrode
Chen, Yan1,2; Yin, Lingling3; Li, YiWei1; Ma, Yaohong1; Yang, Junhui1; Meng, Qingjun1; Shi, Jianguo1
2016-01-22
Source PublicationANALYTICAL LETTERS
Volume49Issue:2Pages:258-268
SubtypeArticle
AbstractA bionanocomposite prepared from multiwalled carbon nanotubes and protamine sulfate is reported for the electrocatalytic determination of dihydronicotinamide adenine dinucleotide. Protamine sulfate was adsorbed via - interactions and hydrogen bonds between protonated amines on arginine and surface groups on the multiwalled carbon nanotubes. The multiwalled carbon nanotube protamine composite accumulated dihydronicotinamide adenine dinucleotide on the electrode surface, resulting in high sensitivity and a low limit of detection for dihydronicotinamide adenine dinucleotide. The nanocomposite was employed for dehydrogenase-based biosensing. Alcohol dehydrogenase was allowed to adsorb on the bionanocomposite surface and the sensitivity of the resulting electrode to ethanol was measured. The alcohol biosensor had an excellent sensitivity of 738.5 mu A/mM with a low limit of detection of 50nM.
KeywordBionanocomposite Dihydronicotinamide Adenine Dinucleotide Oxidation Electrocatalytic Oxidation Ethanol Biosensor Protamine
DOI10.1080/00032719.2015.1065881
Indexed BySCI
Language英语
WOS IDWOS:000364223400008
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/82287
Collection实验海洋生物学重点实验室
Affiliation1.Shandomg Acad Sci, Inst Biol, Key Lab Biosensors Shandong Prov, Jinan 250014, Shandong, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China
3.Shandong Prov Vet Med Supervis Inst, Jinan, Peoples R China
Recommended Citation
GB/T 7714
Chen, Yan,Yin, Lingling,Li, YiWei,et al. Determination of Reduced Nicotinamide Adenine Dinucleotide with a Protamine Multiwalled Carbon Nanotube Electrode[J]. ANALYTICAL LETTERS,2016,49(2):258-268.
APA Chen, Yan.,Yin, Lingling.,Li, YiWei.,Ma, Yaohong.,Yang, Junhui.,...&Shi, Jianguo.(2016).Determination of Reduced Nicotinamide Adenine Dinucleotide with a Protamine Multiwalled Carbon Nanotube Electrode.ANALYTICAL LETTERS,49(2),258-268.
MLA Chen, Yan,et al."Determination of Reduced Nicotinamide Adenine Dinucleotide with a Protamine Multiwalled Carbon Nanotube Electrode".ANALYTICAL LETTERS 49.2(2016):258-268.
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