Pokemon siRNA Delivery Mediated by RGD-Modified HBV Core Protein Suppressed the Growth of Hepatocellular Carcinoma

doi:10.1089/hgtb.2015.093

IOCAS-IR > 实验海洋生物学重点实验室

	Pokemon siRNA Delivery Mediated by RGD-Modified HBV Core Protein Suppressed the Growth of Hepatocellular Carcinoma
	Kong, Jing 1; Liu, Xiaoping 1; Jia, Jianbo 1; Wu, Jinsheng 2; Wu, Ning 3; Chen, Jun 1; Fang, Fang 1
	2015-10-01
发表期刊	HUMAN GENE THERAPY METHODS
卷号	26 期号:5 页码:175-180
文章类型	Article
摘要	Hepatocellular carcinoma (HCC) is a deadly human malignant tumor that is among the most common cancers in the world, especially in Asia. Hepatitis B virus (HBV) infection has been well established as a high risk factor for hepatic malignance. Studies have shown that Pokemon is a master oncogene for HCC growth, suggesting it as an ideal therapeutic target. However, efficient delivery system is still lacking for Pokemon targeting treatment. In this study, we used core proteins of HBV, which is modified with RGD peptides, to construct a biomimetic vector for the delivery of Pokemon siRNAs (namely, RGD-HBc-Pokemon siRNA). Quantitative PCR and Western blot assays revealed that RGD-HBc-Pokemon siRNA possessed the highest efficiency of Pokemon suppression in HCC cells. In vitro experiments further indicated that RGD-HBc-Pokemon-siRNA exerted a higher tumor suppressor activity on HCC cell lines, evidenced by reduced proliferation and attenuated invasiveness, than Pokemon-siRNA or RGD-HBc alone. Finally, animal studies demonstrated that RGD-HBc-Pokemon siRNA suppressed the growth of HCC xenografts in mice by a greater extent than Pokemon-siRNA or RGD-HBc alone. Based on the above results, Pokemon siRNA delivery mediated by RGD-modified HBV core protein was shown to be an effective strategy of HCC gene therapy.
DOI	10.1089/hgtb.2015.093
收录类别	SCI
语种	英语
WOS记录号	WOS:000363943500003
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/82281
专题	实验海洋生物学重点实验室
作者单位	1.Huaiyin Inst Technol, Fac Life Sci & Chem Engn, Huaian 223003, Jiangsu, Peoples R China 2.Nanjing Med Univ, Huaian Peoples Hosp 1, Dept Hepatopancreatobiliary Surg, Huaian, Jiangsu, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Kong, Jing,Liu, Xiaoping,Jia, Jianbo,et al. Pokemon siRNA Delivery Mediated by RGD-Modified HBV Core Protein Suppressed the Growth of Hepatocellular Carcinoma[J]. HUMAN GENE THERAPY METHODS,2015,26(5):175-180.
APA	Kong, Jing.,Liu, Xiaoping.,Jia, Jianbo.,Wu, Jinsheng.,Wu, Ning.,...&Fang, Fang.(2015).Pokemon siRNA Delivery Mediated by RGD-Modified HBV Core Protein Suppressed the Growth of Hepatocellular Carcinoma.HUMAN GENE THERAPY METHODS,26(5),175-180.
MLA	Kong, Jing,et al."Pokemon siRNA Delivery Mediated by RGD-Modified HBV Core Protein Suppressed the Growth of Hepatocellular Carcinoma".HUMAN GENE THERAPY METHODS 26.5(2015):175-180.

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文件名称/大小	文献类型	版本类型	开放类型	使用许可
Pokemon siRNA Delive（437KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览