IOCAS-IR  > 海洋生物技术研发中心
Cloning and characterization of a novel caspase-8-like gene in Crassostrea gigas
Li, Chunyan1,2; Qu, Tao1; Huang, Baoyu1,2; Ji, Peng1,2; Huang, Wen1,2; Que, Huayong1; Li, Li1; Zhang, Guofan1
2015-10-01
发表期刊FISH & SHELLFISH IMMUNOLOGY
卷号46期号:2页码:486-492
文章类型Article
摘要Cysteine-dependent aspartate-directed proteases, or caspases, play key roles in apoptosis and immune defense. In this study, we cloned the first caspase-8-like gene (CgCaspase8-2) identified in the pacific oyster, Crassostrea gigas. The 2572-bp cDNA encodes a putative protein of 714 amino acids that contains two tandem death effector domains (DEDs) at the N-terminal, and P20 and P10 domains at the C-terminal. The conserved pentapeptide motif QACQG was also identified in the deduced CgCaspase8-2 protein. Phylogenetic analysis indicated that CgCaspase8-2 was clustered with initiator caspases in the invertebrate subgroup, but the similarity between CgCaspase8-2 and other invertebrate caspase-8s was low. CgCaspase8-2 protein was localized in the cytoplasm, and over-expression of CgCaspase8-2 in HEK293T cells induced cell death, suggesting a role in apoptosis. Quantitative real-time PCR results demonstrated that CgCaspase8-2 was widely expressed in various tissues and developmental stages, with the highest CgCaspase8-2 expression levels detected in hemolymph and the blastula stage. Furthermore, CgCaspase8-2 transcripts showed no change in response to a bacterial challenge but exhibited notable up-regulation post-poly (I:C) challenge, suggesting that CgCctspase8-2 is specifically involved in immune responses against viruses. In summary, CgCaspase8-2 is involved in both apoptotic and immune function. (C) 2015 Elsevier Ltd. All rights reserved.
关键词Crassostrea Gigas Cgcaspase8-2 Apoptosis Immune Response
DOI10.1016/j.fsi.2015.06.035
收录类别SCI
语种英语
WOS记录号WOS:000363071200037
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/73936
专题海洋生物技术研发中心
实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714
Li, Chunyan,Qu, Tao,Huang, Baoyu,et al. Cloning and characterization of a novel caspase-8-like gene in Crassostrea gigas[J]. FISH & SHELLFISH IMMUNOLOGY,2015,46(2):486-492.
APA Li, Chunyan.,Qu, Tao.,Huang, Baoyu.,Ji, Peng.,Huang, Wen.,...&Zhang, Guofan.(2015).Cloning and characterization of a novel caspase-8-like gene in Crassostrea gigas.FISH & SHELLFISH IMMUNOLOGY,46(2),486-492.
MLA Li, Chunyan,et al."Cloning and characterization of a novel caspase-8-like gene in Crassostrea gigas".FISH & SHELLFISH IMMUNOLOGY 46.2(2015):486-492.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Cloning and characte(848KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Li, Chunyan]的文章
[Qu, Tao]的文章
[Huang, Baoyu]的文章
百度学术
百度学术中相似的文章
[Li, Chunyan]的文章
[Qu, Tao]的文章
[Huang, Baoyu]的文章
必应学术
必应学术中相似的文章
[Li, Chunyan]的文章
[Qu, Tao]的文章
[Huang, Baoyu]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Cloning and characterization of a novel caspase-8-like gene in Crassostrea gigas.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。