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High Specificity of a Quantitative PCR Assay Targeting a Saxitoxin Gene for Monitoring Toxic Algae Associated with Paralytic Shellfish Toxins in the Yellow Sea
Gao, Yan1,2; Yu, Ren-Cheng1; Murray, Shauna A.3; Chen, Jian-Hua1,2; Kang, Zhen-Jun1,2; Zhang, Qing-Chun1; Kong, Fan-Zhou1; Zhou, Ming-Jiang1
2015-10-01
发表期刊APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷号81期号:20页码:6973-6981
文章类型Article
摘要The identification of core genes involved in the biosynthesis of saxitoxin (STX) offers a great opportunity to detect toxic algae associated with paralytic shellfish toxins (PST). In the Yellow Sea (YS) in China, both toxic and nontoxic Alexandrium species are present, which makes it a difficult issue to specifically monitor PST-producing toxic algae. In this study, a quantitative PCR (qPCR) assay targeting sxtA4, a domain in the sxt gene cluster that encodes a unique enzyme involved in STX biosynthesis, was applied to analyze samples collected from the YS in spring of 2012. The abundance of two toxic species within the Alexandrium tamarense species complex, i.e., A. fundyense and A. pacificum, was also determined with TaqMan-based qPCR assays, and PSTs in net-concentrated phytoplankton samples were analyzed with high-performance liquid chromatography coupled with a fluorescence detector. It was found that the distribution of the sxtA4 gene in the YS was consistent with the toxic algae and PSTs, and the quantitation results of sxtA4 correlated well with the abundance of the two toxic species (r = 0.857). These results suggested that the two toxic species were major PST producers during the sampling season and that sxtA-based qPCR is a promising method to detect toxic algae associated with PSTs in the YS. The correlation between PST levels and sxtA-based qPCR results, however, was less significant (r = 0.552), implying that sxtA-based qPCR is not accurate enough to reflect the toxicity of PST-producing toxic algae. The combination of an sxtA-based qPCR assay and chemical means might be a promising method for monitoring toxic algal blooms.
DOI10.1128/AEM.00417-15
收录类别SCI
语种英语
WOS记录号WOS:000363461300004
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/73935
专题海洋生态与环境科学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Univ Technol Sydney, Plant Funct Biol & Climate Change Cluster, Sydney, NSW 2007, Australia
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Gao, Yan,Yu, Ren-Cheng,Murray, Shauna A.,et al. High Specificity of a Quantitative PCR Assay Targeting a Saxitoxin Gene for Monitoring Toxic Algae Associated with Paralytic Shellfish Toxins in the Yellow Sea[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2015,81(20):6973-6981.
APA Gao, Yan.,Yu, Ren-Cheng.,Murray, Shauna A..,Chen, Jian-Hua.,Kang, Zhen-Jun.,...&Zhou, Ming-Jiang.(2015).High Specificity of a Quantitative PCR Assay Targeting a Saxitoxin Gene for Monitoring Toxic Algae Associated with Paralytic Shellfish Toxins in the Yellow Sea.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,81(20),6973-6981.
MLA Gao, Yan,et al."High Specificity of a Quantitative PCR Assay Targeting a Saxitoxin Gene for Monitoring Toxic Algae Associated with Paralytic Shellfish Toxins in the Yellow Sea".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 81.20(2015):6973-6981.
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