Identification of Crassostrea ariakensis and related oysters by multiplex species-specific PCR

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	Identification of Crassostrea ariakensis and related oysters by multiplex species-specific PCR
	Wang, Haiyan 1,2; Guo, Ximing 1
	2008-05-01
发表期刊	JOURNAL OF SHELLFISH RESEARCH
ISSN	0730-8000
卷号	27 期号:3 页码:481-487
文章类型	Article
摘要	Genetic markers are needed for rapid and reliable identification of oysters. In this study, we developed multiplex genus- and species-specific PCR markers for the identification of oysters from China. We used the mitochondrial cytochrome oxidase I (COI) and nuclear 28S ribosomal RNA genes for marker development. DNA sequences from different species were obtained from GenBank or by direct sequencing. Sequences were aligned, and genus- and species-specific nucleotides were identified. Primers were designed for genus/species-specific amplification to generate fragments of different sizes. A multiplex set of genus- and species-specific primers from the 28S gene was able to separate C. ariakensis and C. hongkongensis from other species and assign oysters to four genera. A set of species-specific COI primers provided positive identification of all five Crassostrea species from China, C. ariakensis, C. hongkongensis, C. angulata, C. gigas, and C. sikamea in a single PCR. The multiplex PCR assays do not require fluorescence-labeling or post-PCR enzyme digestion, providing a simple, fast and reliable method for the identification of oysters from China.; Genetic markers are needed for rapid and reliable identification of oysters. In this study, we developed multiplex genus- and species-specific PCR markers for the identification of oysters from China. We used the mitochondrial cytochrome oxidase I (COI) and nuclear 28S ribosomal RNA genes for marker development. DNA sequences from different species were obtained from GenBank or by direct sequencing. Sequences were aligned, and genus- and species-specific nucleotides were identified. Primers were designed for genus/species-specific amplification to generate fragments of different sizes. A multiplex set of genus- and species-specific primers from the 28S gene was able to separate C. ariakensis and C. hongkongensis from other species and assign oysters to four genera. A set of species-specific COI primers provided positive identification of all five Crassostrea species from China, C. ariakensis, C. hongkongensis, C. angulata, C. gigas, and C. sikamea in a single PCR. The multiplex PCR assays do not require fluorescence-labeling or post-PCR enzyme digestion, providing a simple, fast and reliable method for the identification of oysters from China.
关键词	Oyster Identification Species-specific Pcr Cytochrome Oxidase i 28s Rrna Ostreidae Suminoe Oyster Crassostrea Ariakensis
收录类别	SCI
语种	英语
WOS记录号	WOS:000256117200001
引用统计	被引频次：74[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/6330
专题	海洋生物技术研发中心
作者单位	1.Rutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, Piscataway, NJ 08349 USA 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wang, Haiyan,Guo, Ximing. Identification of Crassostrea ariakensis and related oysters by multiplex species-specific PCR[J]. JOURNAL OF SHELLFISH RESEARCH,2008,27(3):481-487.
APA	Wang, Haiyan,&Guo, Ximing.(2008).Identification of Crassostrea ariakensis and related oysters by multiplex species-specific PCR.JOURNAL OF SHELLFISH RESEARCH,27(3),481-487.
MLA	Wang, Haiyan,et al."Identification of Crassostrea ariakensis and related oysters by multiplex species-specific PCR".JOURNAL OF SHELLFISH RESEARCH 27.3(2008):481-487.

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