Effect of ultraviolet irradiation on sperm of the left-eyed flounder, Paralichthys olivaceus

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	Effect of ultraviolet irradiation on sperm of the left-eyed flounder, Paralichthys olivaceus
	You, Feng; Xu, Jianhe; Zhu, Xiangping; Xu, Yongli; Zhang, Peijun
	2008-06-01
发表期刊	JOURNAL OF THE WORLD AQUACULTURE SOCIETY
ISSN	0893-8849
卷号	39 期号:3 页码:414-422
文章类型	Article
摘要	In this study, at proper dosage of ultraviolet (UV) irradiation (180 sec: 36,000 erg/mm(2)), sperm chromosomes of left-eyed flounder, Paralichthys olivaceus, were inactivated, while spermatozoa maintained ability to move and inseminate eggs. Gynogenetic haploids were detected by morphological observation, chromosome counting, and flow cytometer analysis. The ultrastructure of treated sperm was observed under scanning electronic microscope (SEM) and transmission electronic microscope (TEM). The results showed that after being irradiated at lower dosage of irradiation (0-180 sec: 0-36,000 erg/mm(2)), the surface structure of spermatozoa was not affected by UV irradiation, while the inner structures including membrane system and karyoplasm denseness of treated spermatozoa were little changed. However, obvious changes were observed in their membrane system, mitochondria, and nucleus if the dosage of irradiation increased to 240 sec: 48,000 erg/mm(2) or 300 sec: 60,000 erg/mm(2). The sperm survival rates did not change at the lower dosages of the UV irradiation (0-180 sec: 0-36,000 erg/mm(2)) but decreased as the irradiation dosage increased. The motility of treated sperm was lower than that of control group in general but did not change with UV irradiation dosage increasing at the certain range of 0-300 sec: 0-60,000 erg/mm(2).; In this study, at proper dosage of ultraviolet (UV) irradiation (180 sec: 36,000 erg/mm(2)), sperm chromosomes of left-eyed flounder, Paralichthys olivaceus, were inactivated, while spermatozoa maintained ability to move and inseminate eggs. Gynogenetic haploids were detected by morphological observation, chromosome counting, and flow cytometer analysis. The ultrastructure of treated sperm was observed under scanning electronic microscope (SEM) and transmission electronic microscope (TEM). The results showed that after being irradiated at lower dosage of irradiation (0-180 sec: 0-36,000 erg/mm(2)), the surface structure of spermatozoa was not affected by UV irradiation, while the inner structures including membrane system and karyoplasm denseness of treated spermatozoa were little changed. However, obvious changes were observed in their membrane system, mitochondria, and nucleus if the dosage of irradiation increased to 240 sec: 48,000 erg/mm(2) or 300 sec: 60,000 erg/mm(2). The sperm survival rates did not change at the lower dosages of the UV irradiation (0-180 sec: 0-36,000 erg/mm(2)) but decreased as the irradiation dosage increased. The motility of treated sperm was lower than that of control group in general but did not change with UV irradiation dosage increasing at the certain range of 0-300 sec: 0-60,000 erg/mm(2).
关键词	Gynogenesis Induction Spermatozoa Ultrastructure Manipulation Hirame Motility Fishes
收录类别	SCI
语种	英语
WOS记录号	WOS:000256110300011
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/6306
专题	实验海洋生物学重点实验室
作者单位	Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	You, Feng,Xu, Jianhe,Zhu, Xiangping,et al. Effect of ultraviolet irradiation on sperm of the left-eyed flounder, Paralichthys olivaceus[J]. JOURNAL OF THE WORLD AQUACULTURE SOCIETY,2008,39(3):414-422.
APA	You, Feng,Xu, Jianhe,Zhu, Xiangping,Xu, Yongli,&Zhang, Peijun.(2008).Effect of ultraviolet irradiation on sperm of the left-eyed flounder, Paralichthys olivaceus.JOURNAL OF THE WORLD AQUACULTURE SOCIETY,39(3),414-422.
MLA	You, Feng,et al."Effect of ultraviolet irradiation on sperm of the left-eyed flounder, Paralichthys olivaceus".JOURNAL OF THE WORLD AQUACULTURE SOCIETY 39.3(2008):414-422.

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