Expression, purification of recombinant flounder MRF4 protein in Escherichia coli and analysis of its polyclonal antibodies

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	Expression, purification of recombinant flounder MRF4 protein in Escherichia coli and analysis of its polyclonal antibodies
	Xu Peng; Tan Xun-Gang; Zhang Pei-Jun ; Zhang Yu-Qing ; Wang Bo
	2007-11-01
发表期刊	PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
ISSN	1000-3282
卷号	34 期号:11 页码:1169-1174
文章类型	Article
摘要	MRF4 is one of muscle regulatory factors and plays critical roles during skeletal muscle development. The muscle development is important for the fish growth which is an important economic factor for the fish culture. To analyze the function of MRF4 in fish, the founder MRF4 antibody was prepared. The flounder MRF4 was cloned, ligated into prokaryotic expression vector pET-30b and expressed in strain E. coli BL21 (130). The recombinant flounder MRF4 fusion protein was soluble and purified with cobalt IMAC resins. To prepare MRF4 polyclonal antibodies, rabbits were immunized with the soluble protein and the increasing level of antibodies was determined by Western blot. Also, the endogenous flounder MRF4 was recognized by the anti-serum. The result further proved the existence of the anti-MRF4 antibody in the anti-serum, which will be useful for studies on the function of flounder MRF4.; MRF4 is one of muscle regulatory factors and plays critical roles during skeletal muscle development. The muscle development is important for the fish growth which is an important economic factor for the fish culture. To analyze the function of MRF4 in fish, the founder MRF4 antibody was prepared. The flounder MRF4 was cloned, ligated into prokaryotic expression vector pET-30b and expressed in strain E. coli BL21 (130). The recombinant flounder MRF4 fusion protein was soluble and purified with cobalt IMAC resins. To prepare MRF4 polyclonal antibodies, rabbits were immunized with the soluble protein and the increasing level of antibodies was determined by Western blot. Also, the endogenous flounder MRF4 was recognized by the anti-serum. The result further proved the existence of the anti-MRF4 antibody in the anti-serum, which will be useful for studies on the function of flounder MRF4.
关键词	Flounder Expression Purification Mrf4 Antibody
收录类别	SCI
语种	英语
WOS记录号	WOS:000251022800008
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/6304
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Xu Peng,Tan Xun-Gang,Zhang Pei-Jun,et al. Expression, purification of recombinant flounder MRF4 protein in Escherichia coli and analysis of its polyclonal antibodies[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2007,34(11):1169-1174.
APA	Xu Peng,Tan Xun-Gang,Zhang Pei-Jun,Zhang Yu-Qing,&Wang Bo.(2007).Expression, purification of recombinant flounder MRF4 protein in Escherichia coli and analysis of its polyclonal antibodies.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,34(11),1169-1174.
MLA	Xu Peng,et al."Expression, purification of recombinant flounder MRF4 protein in Escherichia coli and analysis of its polyclonal antibodies".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 34.11(2007):1169-1174.

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