IOCAS-IR  > 海洋生物技术研发中心
Effect of long-term cryopreservation on physiological characteristics, antioxidant activities and lipid peroxidation of red seabream (Pagrus major) sperm
Chen, Ya Kun1,2; Liu, Qing Hua1; Li, Jun1; Xiao, Zhi Zhong1; Xu, Shi Hong1; Shi, Xue Hui3; Ma, Dao Yuan1
2010-10-01
发表期刊CRYOBIOLOGY
ISSN0011-2240
卷号61期号:2页码:189-193
文章类型Article
摘要The aim of this study was to determine the effect of long-term cryopreservation on physiological characteristics, the antioxidant activities and lipid peroxidation of red seabream sperm which were respectively cryopreserved with 15% dimethylsulfoxide (Me(2)SO) for 1 month, 13 months, 26 months, 48 months and 73 months. The motility and fertility of post-thaw sperm decreased with the storage time going on. The highest motility (87.67 +/- 2.52%) was obtained in sperm cryopreserved for 1 month and the lowest (50.67 +/- 5.31%) was in sperm for 73 months. There were no significant differences (p < 0.05) in fertilization rates of sperm cryopreserved for 1 month (71.33 +/- 8.84%), 13 months (69.22 +/- 1.02%) and 26 months (60.33 +/- 2.33%); however, the sperm fertility decreased significantly for 48 months (47.22 +/- 3.89%) and 73 months (39.56 +/- 0.69%) storage. In addition, superoxide dismutase (SOD) activities of sperm were at a stable level for less than 26 months storage, then, decreased significantly after 48 months storage. Catalase (CAT) activities of sperm cryopreserved for 13 months, 26 months, 48 months and 73 months were significantly lower than that for 1 month. There were no significant differences in the malondialdehyde (MDA) level of sperm for less than 13 months storage. After 26 months storage, the concentration of MDA increased significantly, and the highest concentration (3.22 +/- 0.05 nmol/mgprot) was obtained in 73 months storage sperm. (C) 2010 Elsevier Inc. All rights reserved.; The aim of this study was to determine the effect of long-term cryopreservation on physiological characteristics, the antioxidant activities and lipid peroxidation of red seabream sperm which were respectively cryopreserved with 15% dimethylsulfoxide (Me2SO) for 1 month, 13 months, 26 months, 48 months and 73 months. The motility and fertility of post-thaw sperm decreased with the storage time going on. The highest motility (87.67 +/- 2.52%) was obtained in sperm cryopreserved for 1 month and the lowest (50.67 +/- 5.31%) was in sperm for 73 months. There were no significant differences (p < 0.05) in fertilization rates of sperm cryopreserved for 1 month (71.33 +/- 8.84%), 13 months (69.22 +/- 1.02%) and 26 months (60.33 +/- 2.33%); however, the sperm fertility decreased significantly for 48 months (47.22 +/- 3.89%) and 73 months (39.56 +/- 0.69%) storage. In addition, superoxide dismutase (SOD) activities of sperm were at a stable level for less than 26 months storage, then, decreased significantly after 48 months storage. Catalase (CAT) activities of sperm cryopreserved for 13 months, 26 months, 48 months and 73 months were significantly lower than that for 1 month. There were no significant differences in the malondialdehyde (MDA) level of sperm for less than 13 months storage. After 26 months storage, the concentration of MDA increased significantly, and the highest concentration (3.22 +/- 0.05 nmol/mgprot) was obtained in 73 months storage sperm. (C) 2010 Elsevier Inc. All rights reserved.
关键词Red Seabream Sperm Long-term Cryopreservation Physiological Characteristics Antioxidant Activities Lipid Peroxidation
学科领域Biology ; Physiology
DOI10.1016/j.cryobiol.2010.07.003
URL查看原文
收录类别SCI
语种英语
WOS记录号WOS:000282716800004
引用统计
被引频次:38[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/6138
专题海洋生物技术研发中心
作者单位1.Chinese Acad Sci, Ctr Biotechnol R&D, Inst Oceanol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
3.Qingdao Agr Univ, Qingdao 266109, Peoples R China
第一作者单位中国科学院海洋研究所
推荐引用方式
GB/T 7714
Chen, Ya Kun,Liu, Qing Hua,Li, Jun,et al. Effect of long-term cryopreservation on physiological characteristics, antioxidant activities and lipid peroxidation of red seabream (Pagrus major) sperm[J]. CRYOBIOLOGY,2010,61(2):189-193.
APA Chen, Ya Kun.,Liu, Qing Hua.,Li, Jun.,Xiao, Zhi Zhong.,Xu, Shi Hong.,...&Ma, Dao Yuan.(2010).Effect of long-term cryopreservation on physiological characteristics, antioxidant activities and lipid peroxidation of red seabream (Pagrus major) sperm.CRYOBIOLOGY,61(2),189-193.
MLA Chen, Ya Kun,et al."Effect of long-term cryopreservation on physiological characteristics, antioxidant activities and lipid peroxidation of red seabream (Pagrus major) sperm".CRYOBIOLOGY 61.2(2010):189-193.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Effect of long-term (322KB) 限制开放--浏览
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Chen, Ya Kun]的文章
[Liu, Qing Hua]的文章
[Li, Jun]的文章
百度学术
百度学术中相似的文章
[Chen, Ya Kun]的文章
[Liu, Qing Hua]的文章
[Li, Jun]的文章
必应学术
必应学术中相似的文章
[Chen, Ya Kun]的文章
[Liu, Qing Hua]的文章
[Li, Jun]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Effect of long-term cryopreservation on physiological characteristics, antioxidant activities and lipid peroxidation of red seabream (Pagrus major) sperm.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。