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Use of computer-assisted sperm analysis (CASA) to evaluate the quality of cryopreserved sperm in red seabream (Pagrus major)
Liu, Qing Hua; Li, Jun; Xiao, Zhi Zhong; Ding, Fu Hong; Yu, Dao De; Xu, Xi Zhang
2007-03-06
发表期刊AQUACULTURE
ISSN0044-8486
卷号263期号:1-4页码:20-25
文章类型Article
摘要In the present study, the quality of post-thaw sperm of red seabream Pagrus major frozen with 6-24% DMSO was investigated. The motility, average path velocity and fertilizing capacity of fresh and their corresponding post-thaw sperm were examined for evaluation of the post-thaw sperm motion characteristics and its association with fertilizing capacity. An analysis of sperm motility before and after cryopreservation has been performed using computer-assisted sperm analysis (CASA). For post-thaw sperm frozen with 12-21% DMSO, the percentages of motile sperm were not significantly (P > 0.05) changed 10 s after activation. Moreover, the main motility pattern and swimming velocity of the motile post-thaw sperm were not significantly (P > 0.05) changed and the progressive linear motion was still the dominant pattern. However, the total motility of post-thaw sperm (72.3 +/- 6.3%) 30 s after activation was (P < 0.05) lower than the corresponding fresh sperm (82.7 +/- 7.2%). Additionally, the fertilizing capacity of post-thaw sperm was investigated with a standardized sperm to egg ratio 500:1. There is a linear regression relationship between the percentage of motile post-thaw sperm and fertilizing capability. These data demonstrate that 12-21% DMSO can provide good protection to the sperm during the freezing-thawing process. (c) 2006 Elsevier B.V. All rights reserved.; In the present study, the quality of post-thaw sperm of red seabream Pagrus major frozen with 6-24% DMSO was investigated. The motility, average path velocity and fertilizing capacity of fresh and their corresponding post-thaw sperm were examined for evaluation of the post-thaw sperm motion characteristics and its association with fertilizing capacity. An analysis of sperm motility before and after cryopreservation has been performed using computer-assisted sperm analysis (CASA). For post-thaw sperm frozen with 12-21% DMSO, the percentages of motile sperm were not significantly (P > 0.05) changed 10 s after activation. Moreover, the main motility pattern and swimming velocity of the motile post-thaw sperm were not significantly (P > 0.05) changed and the progressive linear motion was still the dominant pattern. However, the total motility of post-thaw sperm (72.3 +/- 6.3%) 30 s after activation was (P < 0.05) lower than the corresponding fresh sperm (82.7 +/- 7.2%). Additionally, the fertilizing capacity of post-thaw sperm was investigated with a standardized sperm to egg ratio 500:1. There is a linear regression relationship between the percentage of motile post-thaw sperm and fertilizing capability. These data demonstrate that 12-21% DMSO can provide good protection to the sperm during the freezing-thawing process. (c) 2006 Elsevier B.V. All rights reserved.
关键词Casa Red Seabream Pagrus Major Sperm Motility Velocity Fertilizing Capacity
学科领域Fisheries ; Marine & Freshwater Biology
DOI10.1016/j.aquaculture.2006.11.017
URL查看原文
收录类别SCI
语种英语
WOS记录号WOS:000244547600003
引用统计
被引频次:42[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/6070
专题海洋生物技术研发中心
作者单位1.Chinese Acad Sci, Inst Oceanol, Ctr Marine Biotechnol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100031, Peoples R China
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GB/T 7714
Liu, Qing Hua,Li, Jun,Xiao, Zhi Zhong,et al. Use of computer-assisted sperm analysis (CASA) to evaluate the quality of cryopreserved sperm in red seabream (Pagrus major)[J]. AQUACULTURE,2007,263(1-4):20-25.
APA Liu, Qing Hua,Li, Jun,Xiao, Zhi Zhong,Ding, Fu Hong,Yu, Dao De,&Xu, Xi Zhang.(2007).Use of computer-assisted sperm analysis (CASA) to evaluate the quality of cryopreserved sperm in red seabream (Pagrus major).AQUACULTURE,263(1-4),20-25.
MLA Liu, Qing Hua,et al."Use of computer-assisted sperm analysis (CASA) to evaluate the quality of cryopreserved sperm in red seabream (Pagrus major)".AQUACULTURE 263.1-4(2007):20-25.
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