Flow cytometry and ultrastructure of cryopreserved red seabream (Pagrus major) sperm

doi:10.1016/j.theriogenology.2006.12.013

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	Flow cytometry and ultrastructure of cryopreserved red seabream (Pagrus major) sperm
	Liu, Q. H.; Li, J.; Zhang, S. C.; Xiao, Z. Z.; Ding, F. H.; Yu, D. D.; Xu, X. Z.
	2007-04-01
发表期刊	THERIOGENOLOGY
ISSN	0093-691X
卷号	67 期号:6 页码:1168-1174
文章类型	Article
摘要	The objectives were to assess motility, fertilizing capacity, structural integrity, and mitochondrial function in fresh versus frozen-thawed (15% DMSO was used as a cryoprotectant) sperm from red seabrearn (Pagrus major). Mean (+/- S.D.) rates of motility, fertilization and hatching of frozen-thawed sperm were 81.0 +/- 5.4, 92.8 +/- 1.9, and 91.8 +/- 5.2%, respectively; for fresh sperm, they were 87.5 +/- 7.7, 95.8 +/- 2.4, and 93.8 +/- 4.2%. Although motility was lower in frozen-thawed versus fresh sperm (P < 0.05), there was no effect (P > 0.05) of cryopreservation on fertilization or hatching. Based on scanning and transmission electron microscopy, 77.8 +/- 5.6% of fresh sperm had normal morphology, whereas for frozen-thawed sperm, 63.0 +/- 7.2% had normal morphology, 20.6 +/- 3.1% were slightly damaged (e.g. swelling or rupture of head, mid-piece and tail region as well as mitochondria), and 16.4 +/- 4.2% were severely damaged. Sperm were stained with propidium iodide and Rhodamine 123 to assess plasma membrane integrity and mitochondrial function, respectively, and examined with flow cytometry. For fresh sperm, 83.9% had an intact membrane and functional mitochondria, whereas for frozen-thawed sperm, 74.8% had an intact membrane and functional mitochondria, 12.7% had a damaged membrane, 9.9% had nonfunctional mitochondria, and 2.6% had both a damaged membrane and nonfunctional mitochondria. In conclusion, ultrastructure and flow cytometry were valuable for assessment of frozen-thawed sperm quality; cryopreservation damaged the sperm but fertilizing ability was not significantly decreased. (c) 2007 Elsevier Inc. All rights reserved.; The objectives were to assess motility, fertilizing capacity, structural integrity, and mitochondrial function in fresh versus frozen-thawed (15% DMSO was used as a cryoprotectant) sperm from red seabrearn (Pagrus major). Mean (+/- S.D.) rates of motility, fertilization and hatching of frozen-thawed sperm were 81.0 +/- 5.4, 92.8 +/- 1.9, and 91.8 +/- 5.2%, respectively; for fresh sperm, they were 87.5 +/- 7.7, 95.8 +/- 2.4, and 93.8 +/- 4.2%. Although motility was lower in frozen-thawed versus fresh sperm (P < 0.05), there was no effect (P > 0.05) of cryopreservation on fertilization or hatching. Based on scanning and transmission electron microscopy, 77.8 +/- 5.6% of fresh sperm had normal morphology, whereas for frozen-thawed sperm, 63.0 +/- 7.2% had normal morphology, 20.6 +/- 3.1% were slightly damaged (e.g. swelling or rupture of head, mid-piece and tail region as well as mitochondria), and 16.4 +/- 4.2% were severely damaged. Sperm were stained with propidium iodide and Rhodamine 123 to assess plasma membrane integrity and mitochondrial function, respectively, and examined with flow cytometry. For fresh sperm, 83.9% had an intact membrane and functional mitochondria, whereas for frozen-thawed sperm, 74.8% had an intact membrane and functional mitochondria, 12.7% had a damaged membrane, 9.9% had nonfunctional mitochondria, and 2.6% had both a damaged membrane and nonfunctional mitochondria. In conclusion, ultrastructure and flow cytometry were valuable for assessment of frozen-thawed sperm quality; cryopreservation damaged the sperm but fertilizing ability was not significantly decreased. (c) 2007 Elsevier Inc. All rights reserved.
关键词	Cryopreservation Flow Cytometry Red Seabream Sperm Ultrastructure
学科领域	Reproductive Biology ; Veterinary Sciences
DOI	10.1016/j.theriogenology.2006.12.013
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000245422700008
引用统计	被引频次：22[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/6064
专题	海洋生物技术研发中心实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Biotechnol, Res & Dev, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100031, Peoples R China 3.Ocean Univ China, Dept Marine Biol, Qingdao 266003, Peoples R China
推荐引用方式 GB/T 7714	Liu, Q. H.,Li, J.,Zhang, S. C.,et al. Flow cytometry and ultrastructure of cryopreserved red seabream (Pagrus major) sperm[J]. THERIOGENOLOGY,2007,67(6):1168-1174.
APA	Liu, Q. H..,Li, J..,Zhang, S. C..,Xiao, Z. Z..,Ding, F. H..,...&Xu, X. Z..(2007).Flow cytometry and ultrastructure of cryopreserved red seabream (Pagrus major) sperm.THERIOGENOLOGY,67(6),1168-1174.
MLA	Liu, Q. H.,et al."Flow cytometry and ultrastructure of cryopreserved red seabream (Pagrus major) sperm".THERIOGENOLOGY 67.6(2007):1168-1174.

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