| Effect of ethanol extract of alga Laurencia supplementation on DNA oxidation and alkylation damage in mice | |
| Liang, Hui; He, Juan; Ma, Ai-guo; Zhang, Pei-hua; Bi, Shu-lian; Shi, Da-yong | |
| 2007 | |
| 发表期刊 | ASIA PACIFIC JOURNAL OF CLINICAL NUTRITION
 |
| ISSN | 0964-7058 |
| 卷号 | 16页码:164-168 |
| 文章类型 | Article |
| 摘要 | Laurencia terpenoid extract (LET) had been extracted from the red alga Laurencia tristicha. The study is to investigate the effects of LET supplementation on DNA oxidation and alkylation damages in mice. Forty healthy kunming mice weighing between 18g and 25g were randomly assigned into 4 groups, each consisting of ten animals. The mice were orally intubated respectively for 60 days with the designed concentrations of LET (25, 50, 100 mg/kg b.w.) for three exposed groups and salad oil (0.2 ml) for the blank group. Food and water were free for the animals. Mice in the blank and exposed groups were sacrificed after the last treatment and the blood of each animal was quickly taken for further experiments. The spontaneous and oxidized DNA damages of peripheral lymphocytes induced by H2O2 were analysed by SCGE. O-6-Methy-guanine (O-6-MeG) was measured by high performance capillary zone electrophoresis. There was no significantly difference in DNA spontaneous damage on peripheral lymphocytes of all the mice. The oxidative DNA damage in the 50 mg/Kg body weight supplement group are 286AU with the oxidation of 10 mu mol/L H2O2, significantly lower than the blank group 332AU (p<0.05). The contents of O-6-MeG in plasma in the 50mg/kg b.w. and 100mg/kg b.w. supplement group were 1.50 mu mol/L andl.88 mu mol/L, significantly lower than that of the blank group, which was 2.89 mu mol/L(p<0.05). The results from the present study indicated that the LET were rich in terpenoids and safety to be taken orally and it could improve antioxidative and decrease DNA damage effectively.; Laurencia terpenoid extract (LET) had been extracted from the red alga Laurencia tristicha. The study is to investigate the effects of LET supplementation on DNA oxidation and alkylation damages in mice. Forty healthy kunming mice weighing between 18g and 25g were randomly assigned into 4 groups, each consisting of ten animals. The mice were orally intubated respectively for 60 days with the designed concentrations of LET (25, 50, 100 mg/kg b.w.) for three exposed groups and salad oil (0.2 ml) for the blank group. Food and water were free for the animals. Mice in the blank and exposed groups were sacrificed after the last treatment and the blood of each animal was quickly taken for further experiments. The spontaneous and oxidized DNA damages of peripheral lymphocytes induced by H2O2 were analysed by SCGE. O-6-Methy-guanine (O-6-MeG) was measured by high performance capillary zone electrophoresis. There was no significantly difference in DNA spontaneous damage on peripheral lymphocytes of all the mice. The oxidative DNA damage in the 50 mg/Kg body weight supplement group are 286AU with the oxidation of 10 mu mol/L H2O2, significantly lower than the blank group 332AU (p<0.05). The contents of O-6-MeG in plasma in the 50mg/kg b.w. and 100mg/kg b.w. supplement group were 1.50 mu mol/L andl.88 mu mol/L, significantly lower than that of the blank group, which was 2.89 mu mol/L(p<0.05). The results from the present study indicated that the LET were rich in terpenoids and safety to be taken orally and it could improve antioxidative and decrease DNA damage effectively. |
| 关键词 | Laurencia Terpenoid Scge O-6-methylguanine Dna Damage |
| 学科领域 | Nutrition & Dietetics |
| 收录类别 | ISTP ; SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000245926500031 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/6060 |
| 专题 | 海洋生物技术研发中心 |
| 作者单位 | 1.Qingdao Univ, Coll Med, Inst Human Nutr, Shandong 266021, Peoples R China 2.Ocean Univ China, Dept Marine Biol, Qingdao 266003, Peoples R China 3.Qingdao Univ, Coll Med, Affiliated Hosp, Dept Nutr, Shandong 266021, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liang, Hui,He, Juan,Ma, Ai-guo,et al. Effect of ethanol extract of alga Laurencia supplementation on DNA oxidation and alkylation damage in mice[J]. ASIA PACIFIC JOURNAL OF CLINICAL NUTRITION,2007,16:164-168. |
| APA | Liang, Hui,He, Juan,Ma, Ai-guo,Zhang, Pei-hua,Bi, Shu-lian,&Shi, Da-yong.(2007).Effect of ethanol extract of alga Laurencia supplementation on DNA oxidation and alkylation damage in mice.ASIA PACIFIC JOURNAL OF CLINICAL NUTRITION,16,164-168. |
| MLA | Liang, Hui,et al."Effect of ethanol extract of alga Laurencia supplementation on DNA oxidation and alkylation damage in mice".ASIA PACIFIC JOURNAL OF CLINICAL NUTRITION 16(2007):164-168. |
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