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Expression of beta-carotene hydroxylase gene (crtR-B) from the green alga Haematococcus pluvialis in chloroplasts of Chlamydomonas reinhardtii
Tan, Cong-Ping; Zhao, Fang-Qing; Su, Zhong-Liang; Liang, Cheng-Wei; Qin, Song
2007-08-01
发表期刊JOURNAL OF APPLIED PHYCOLOGY
ISSN0921-8971
卷号19期号:4页码:347-355
文章类型Article
摘要A carotenoid gene (crtR-B) from the green alga Haematococcus pluvialis, encoding beta-carotene hydroxylase that was able to catalyze the conversion of beta-carotene to zeaxanthin and canthaxanthin to astaxanthin, was cloned into Chlamydomonas reinhardtii chloroplast expression vector p64D to yield plasmid p64DcrtR-B. The vector p64DcrtR-B was transferred to the chloroplast genome of C. reinhardtii using micro-particle bombardment. PCR and Southern blot analyses indicated that crtR-B was integrated into the chloroplast genome of the transformants. RTPCR assays showed that the H. pluvialis crt R-B gene was expressed in C. reinhardtii transformants. The transformants rapidly synthesized carotenoids in larger quantities than the wild-type upon being transferred from moderate to high-intensity white light. This research provides a foundation for further study to elucidate the possible mechanism of photo-protection by xanthophylls and other carotenoids in high light conditions or through exposure to UV radiation.; A carotenoid gene (crtR-B) from the green alga Haematococcus pluvialis, encoding beta-carotene hydroxylase that was able to catalyze the conversion of beta-carotene to zeaxanthin and canthaxanthin to astaxanthin, was cloned into Chlamydomonas reinhardtii chloroplast expression vector p64D to yield plasmid p64DcrtR-B. The vector p64DcrtR-B was transferred to the chloroplast genome of C. reinhardtii using micro-particle bombardment. PCR and Southern blot analyses indicated that crtR-B was integrated into the chloroplast genome of the transformants. RTPCR assays showed that the H. pluvialis crt R-B gene was expressed in C. reinhardtii transformants. The transformants rapidly synthesized carotenoids in larger quantities than the wild-type upon being transferred from moderate to high-intensity white light. This research provides a foundation for further study to elucidate the possible mechanism of photo-protection by xanthophylls and other carotenoids in high light conditions or through exposure to UV radiation.
关键词Chlamydomonas Reinhardtii Chloroplast Transformation Beta-carotene Hydroxylase Gene (Crtr-b) Carotenoids
学科领域Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
DOI10.1007/s10811-006-9141-8
URL查看原文
收录类别SCI
语种英语
WOS记录号WOS:000248188000006
引用统计
被引频次:24[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/5994
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Shandong Inst Light Ind, Coll Food & Bioengn, Jinan 250100, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
4.Ocean Univ Sci & Technol, Coll Chem Engn, Qingdao 266042, Peoples R China
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GB/T 7714
Tan, Cong-Ping,Zhao, Fang-Qing,Su, Zhong-Liang,et al. Expression of beta-carotene hydroxylase gene (crtR-B) from the green alga Haematococcus pluvialis in chloroplasts of Chlamydomonas reinhardtii[J]. JOURNAL OF APPLIED PHYCOLOGY,2007,19(4):347-355.
APA Tan, Cong-Ping,Zhao, Fang-Qing,Su, Zhong-Liang,Liang, Cheng-Wei,&Qin, Song.(2007).Expression of beta-carotene hydroxylase gene (crtR-B) from the green alga Haematococcus pluvialis in chloroplasts of Chlamydomonas reinhardtii.JOURNAL OF APPLIED PHYCOLOGY,19(4),347-355.
MLA Tan, Cong-Ping,et al."Expression of beta-carotene hydroxylase gene (crtR-B) from the green alga Haematococcus pluvialis in chloroplasts of Chlamydomonas reinhardtii".JOURNAL OF APPLIED PHYCOLOGY 19.4(2007):347-355.
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