Cloning, expression and identification of ferritin from Chinese shrimp, Fenneropenaeus chinensis

doi:10.1016/j.jbiotec.2006.03.010

IOCAS-IR > 实验海洋生物学重点实验室

	Cloning, expression and identification of ferritin from Chinese shrimp, Fenneropenaeus chinensis
	Zhang, Jiquan ; Li, Fuhua ; Wang, Zaizhao ; Zhang, Xiaojun ; Zhou, Qian ; Xiang, Jianhai
	2006-09-01
发表期刊	JOURNAL OF BIOTECHNOLOGY
ISSN	0168-1656
卷号	125 期号:2 页码:173-184
文章类型	Article
摘要	Ferritin, the iron storage protein, plays a key role in iron metabolism. A cDNA encoding ferritin (FcFer) was cloned from hepatopancreas of Chinese shrimp, Fenneropenaeus chinensis. The predicted protein contains 170 amino acid residues with a predicted molecular weight (MW) about 19, 422.89 Da and theoretical isoelectric point (PI) of 4.73. Amino acid alignment of FcFer revealed 97% homology with Litopenaeus vannamei ferritin. Results of the RT-PCR showed that the expression of FcFer mRNA was up-regulated after shrimp was challenged with either white spot syndrome virus (WSSV) or heavy metal ions (Zn2+ and Cu2+) in the laboratory. A fusion protein containing FcFer was produced and the purified recombinant protein exhibited similar function of iron uptake in vitro. The result of in-gel digestion and identification using LC-ESI-MS showed that two peptide fragments (-DDVALPGFAK- and -LLEDEYLEEQVDS1KK-) of the recombinant protein were identical to the corresponding sequence of L. vannamei ferritin. The recombinant FcFer protein will be proved useful for study on the structure and function of ferritin in F chinensis. (c) 2006 Elsevier B.V. All rights reserved.; Ferritin, the iron storage protein, plays a key role in iron metabolism. A cDNA encoding ferritin (FcFer) was cloned from hepatopancreas of Chinese shrimp, Fenneropenaeus chinensis. The predicted protein contains 170 amino acid residues with a predicted molecular weight (MW) about 19, 422.89 Da and theoretical isoelectric point (PI) of 4.73. Amino acid alignment of FcFer revealed 97% homology with Litopenaeus vannamei ferritin. Results of the RT-PCR showed that the expression of FcFer mRNA was up-regulated after shrimp was challenged with either white spot syndrome virus (WSSV) or heavy metal ions (Zn2+ and Cu2+) in the laboratory. A fusion protein containing FcFer was produced and the purified recombinant protein exhibited similar function of iron uptake in vitro. The result of in-gel digestion and identification using LC-ESI-MS showed that two peptide fragments (-DDVALPGFAK- and -LLEDEYLEEQVDS1KK-) of the recombinant protein were identical to the corresponding sequence of L. vannamei ferritin. The recombinant FcFer protein will be proved useful for study on the structure and function of ferritin in F chinensis. (c) 2006 Elsevier B.V. All rights reserved.
关键词	Fenneropenaeus Chinensis Ferritin White Spot Syndrome Virus Semi-quantitative Rt-pcr Metal Ions
学科领域	Biotechnology & Applied Microbiology
DOI	10.1016/j.jbiotec.2006.03.010
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000240208800002
引用统计	被引频次：70[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5976
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Jiquan,Li, Fuhua,Wang, Zaizhao,et al. Cloning, expression and identification of ferritin from Chinese shrimp, Fenneropenaeus chinensis[J]. JOURNAL OF BIOTECHNOLOGY,2006,125(2):173-184.
APA	Zhang, Jiquan,Li, Fuhua,Wang, Zaizhao,Zhang, Xiaojun,Zhou, Qian,&Xiang, Jianhai.(2006).Cloning, expression and identification of ferritin from Chinese shrimp, Fenneropenaeus chinensis.JOURNAL OF BIOTECHNOLOGY,125(2),173-184.
MLA	Zhang, Jiquan,et al."Cloning, expression and identification of ferritin from Chinese shrimp, Fenneropenaeus chinensis".JOURNAL OF BIOTECHNOLOGY 125.2(2006):173-184.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
zhang-Cloning, expre（995KB）			限制开放	--	浏览