IOCAS-IR  > 实验海洋生物学重点实验室
The first molluscan TCTP in Venerupis philippinarum: Molecular cloning and expression analysis
Li, Chenghua1; Qiu, Limei2; Ning, Xuanxuan3; Chen, Aiqin1; Qin, Song1; Wu, Huifeng1; Zhao, Jianmin1
2010-09-01
发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
卷号29期号:3页码:530-533
文章类型Article
摘要Translationally controlled tumor protein (TCTP) is one of the abundant and ubiquitously expressed proteins in metazoans In the present study, the first molluscan TCTP (denoted as VpTCTP) was identified from Venerupis philippinarum haemocytes by EST and RACE approaches The full-length cDNA of VpTCTP consisted of 1148 nucleotides with an open-reading frame of 555 bp encoding 184 amino acids The deduced amino acid sequence of VpTCTP shared high similarity with TCTPs from other species, indicating that VpTCTP should be a new member of TCTP family Several highly conserved motifs, including 5'terminal ologopyrimidine (5'TOP) starting sequence and rich AU and AUUT elements in 3'UTR, were also identified in VpTCTP The tissue and temporal expression of VpTCTP after Vi boo anguillarum challenge was recorded by quantitative real-time RT-PCR. VpTCTP transcript could be detected in all examined tissues with the highest expression level in haemocytes and the lowest in hepatopancreas Concerning the time-course expression in haemocytes, the relative expression of VpTCTP mRNA was down-regulated sharply from 6 h to 12 h post-infection. Then, the expression level was obviously up-regulated and reached 3.4-fold to that in the control group at 48 h post challenge As time progressed, the expression of VpTCTP recovered to the original level at 96 h. All these results indicated that VpTCTP was an acute-phase protein involved in the Immune response of V philippinarum (C) 2010 Elsevier Ltd. All rights reserved.; Translationally controlled tumor protein (TCTP) is one of the abundant and ubiquitously expressed proteins in metazoans In the present study, the first molluscan TCTP (denoted as VpTCTP) was identified from Venerupis philippinarum haemocytes by EST and RACE approaches The full-length cDNA of VpTCTP consisted of 1148 nucleotides with an open-reading frame of 555 bp encoding 184 amino acids The deduced amino acid sequence of VpTCTP shared high similarity with TCTPs from other species, indicating that VpTCTP should be a new member of TCTP family Several highly conserved motifs, including 5'terminal ologopyrimidine (5'TOP) starting sequence and rich AU and AUUT elements in 3'UTR, were also identified in VpTCTP The tissue and temporal expression of VpTCTP after Vi boo anguillarum challenge was recorded by quantitative real-time RT-PCR. VpTCTP transcript could be detected in all examined tissues with the highest expression level in haemocytes and the lowest in hepatopancreas Concerning the time-course expression in haemocytes, the relative expression of VpTCTP mRNA was down-regulated sharply from 6 h to 12 h post-infection. Then, the expression level was obviously up-regulated and reached 3.4-fold to that in the control group at 48 h post challenge As time progressed, the expression of VpTCTP recovered to the original level at 96 h. All these results indicated that VpTCTP was an acute-phase protein involved in the Immune response of V philippinarum (C) 2010 Elsevier Ltd. All rights reserved.
关键词Venerupis Philippinarum Tctp Tissues Expression Time-course Expression
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI10.1016/j.fsi.2010.04.018
URL查看原文
收录类别SCI
语种英语
WOS记录号WOS:000280513700020
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/5942
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
3.SOA, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China
推荐引用方式
GB/T 7714
Li, Chenghua,Qiu, Limei,Ning, Xuanxuan,et al. The first molluscan TCTP in Venerupis philippinarum: Molecular cloning and expression analysis[J]. FISH & SHELLFISH IMMUNOLOGY,2010,29(3):530-533.
APA Li, Chenghua.,Qiu, Limei.,Ning, Xuanxuan.,Chen, Aiqin.,Qin, Song.,...&Zhao, Jianmin.(2010).The first molluscan TCTP in Venerupis philippinarum: Molecular cloning and expression analysis.FISH & SHELLFISH IMMUNOLOGY,29(3),530-533.
MLA Li, Chenghua,et al."The first molluscan TCTP in Venerupis philippinarum: Molecular cloning and expression analysis".FISH & SHELLFISH IMMUNOLOGY 29.3(2010):530-533.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
The first molluscan (451KB) 限制开放--浏览
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Li, Chenghua]的文章
[Qiu, Limei]的文章
[Ning, Xuanxuan]的文章
百度学术
百度学术中相似的文章
[Li, Chenghua]的文章
[Qiu, Limei]的文章
[Ning, Xuanxuan]的文章
必应学术
必应学术中相似的文章
[Li, Chenghua]的文章
[Qiu, Limei]的文章
[Ning, Xuanxuan]的文章
相关权益政策
暂无数据
收藏/分享
文件名: The first molluscan TCTP in Venerupis philippinarum_ Molecular cloning and expression analysis.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。