The recombination and expression of the allophycocyanin beta subunit gene in the chloroplast of Chlamydomonas reinhardtii

doi:10.1007/s11274-005-9001-8

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	The recombination and expression of the allophycocyanin beta subunit gene in the chloroplast of Chlamydomonas reinhardtii
	Su, ZL ; He, DM ; Qian, KX ; Zhao, FQ ; Meng, CX ; Qin, S
	2006-02-01
发表期刊	WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
ISSN	0959-3993
卷号	22 期号:2 页码:101-103
文章类型	Article
摘要	A Chlamydomonas reinhardtii (C. reinhardtii) chloroplast expression vector, papc-B, containing the apc-B gene that encodes the beta subunit of the light-harvesting antenna protein allophycocyanin (APC) of cyanobacteria, was constructed and transferred to the chloroplast genome of C. reinhardtii by the biolistic method. The transformants were identified by Southern blot, Western blot and ELISA assays after selection on resistant medium. The recombinant APC beta subunit was expressed in the C. reinhardtii chloroplast and accounted for up to 2-3% (w/w) of the total soluble protein (TSP), suggesting a promising prospect of using C. reinhardtii chloroplasts to produce functional plant-derived proteins.; A Chlamydomonas reinhardtii (C. reinhardtii) chloroplast expression vector, papc-B, containing the apc-B gene that encodes the beta subunit of the light-harvesting antenna protein allophycocyanin (APC) of cyanobacteria, was constructed and transferred to the chloroplast genome of C. reinhardtii by the biolistic method. The transformants were identified by Southern blot, Western blot and ELISA assays after selection on resistant medium. The recombinant APC beta subunit was expressed in the C. reinhardtii chloroplast and accounted for up to 2-3% (w/w) of the total soluble protein (TSP), suggesting a promising prospect of using C. reinhardtii chloroplasts to produce functional plant-derived proteins.
关键词	Allophycocyanin Beta Subunit Gene Chlamydomonas Reinhardtii Chloroplast Transformation
学科领域	Biotechnology & Applied Microbiology
DOI	10.1007/s11274-005-9001-8
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000236077700002
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5928
专题	实验海洋生物学重点实验室
作者单位	1.Zhejiang Univ, Coll Life Sci, Dept Biotechnol, Hangzhou 310027, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Su, ZL,He, DM,Qian, KX,et al. The recombination and expression of the allophycocyanin beta subunit gene in the chloroplast of Chlamydomonas reinhardtii[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2006,22(2):101-103.
APA	Su, ZL,He, DM,Qian, KX,Zhao, FQ,Meng, CX,&Qin, S.(2006).The recombination and expression of the allophycocyanin beta subunit gene in the chloroplast of Chlamydomonas reinhardtii.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,22(2),101-103.
MLA	Su, ZL,et al."The recombination and expression of the allophycocyanin beta subunit gene in the chloroplast of Chlamydomonas reinhardtii".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 22.2(2006):101-103.

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