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Development of expressed sequence tags from the bay scallop, Argopecten irradians irradians
Song, LS; Xu, W; Li, CH; Li, HL; Wu, LT; Xiang, JH; Guo, XM
2006-04-01
发表期刊MARINE BIOTECHNOLOGY
ISSN1436-2228
卷号8期号:2页码:161-169
文章类型Article
摘要The bay scallop, Argopecten irradians irradians, introduced from North America, has become one of the most important aquaculture species in China. Inan effort to identify scallop genes involved in host defense, a high-quality cDNA library was constructed from whole body tissues of the bay scallop. A total of 5828 successful sequencing reactions yielded 4995 expressed sequence tags (ESTs) longer than 100 bp. Cluster and assembly analyses of the ESTs identified 637 contigs (consisting of 2853 sequences) and 2142 singletons, totaling 2779 unique sequences. Basic Local Alignment Search Tool (BLAST) analysis showed that the majority (73%) of the unique sequences had no significant homology (E-value >= 0.005) to sequences in GenBank. Among the 748 sequences with significant GenBank matches, 160 (21.4%) were for genes related to metabolism, 131 (17.5%) for cell/organism defense, 124 (16.6%) for gene/protein expression, 83 (11.1%) for cell structure/motility, 70 (9.4%) for cell signaling/communication, 17 (2.3%) for cell division, and 163 (21.8%) matched to genes of unknown functions. The list of host-defense genes included many genes with known and important roles in innate defense such as lectins, defensins, proteases, protease inhibitors, heat shock proteins, antioxidants, and Toll-like receptors. The study provides a significant number of ESTs for gene discovery and candidate genes for studying host defense in scallops and other molluscs.; The bay scallop, Argopecten irradians irradians, introduced from North America, has become one of the most important aquaculture species in China. Inan effort to identify scallop genes involved in host defense, a high-quality cDNA library was constructed from whole body tissues of the bay scallop. A total of 5828 successful sequencing reactions yielded 4995 expressed sequence tags (ESTs) longer than 100 bp. Cluster and assembly analyses of the ESTs identified 637 contigs (consisting of 2853 sequences) and 2142 singletons, totaling 2779 unique sequences. Basic Local Alignment Search Tool (BLAST) analysis showed that the majority (73%) of the unique sequences had no significant homology (E-value >= 0.005) to sequences in GenBank. Among the 748 sequences with significant GenBank matches, 160 (21.4%) were for genes related to metabolism, 131 (17.5%) for cell/organism defense, 124 (16.6%) for gene/protein expression, 83 (11.1%) for cell structure/motility, 70 (9.4%) for cell signaling/communication, 17 (2.3%) for cell division, and 163 (21.8%) matched to genes of unknown functions. The list of host-defense genes included many genes with known and important roles in innate defense such as lectins, defensins, proteases, protease inhibitors, heat shock proteins, antioxidants, and Toll-like receptors. The study provides a significant number of ESTs for gene discovery and candidate genes for studying host defense in scallops and other molluscs.
关键词Argopecten Irradians Bay Scallop Cdna Library Diseases Expressed Sequence Tags Host Defense
学科领域Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
DOI10.1007/s10126-005-0126-4
URL查看原文
收录类别SCI
语种英语
WOS记录号WOS:000236968600007
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被引频次:80[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/5918
专题实验海洋生物学重点实验室
作者单位1.Rutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, Port Norris, NJ 08349 USA
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
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Song, LS,Xu, W,Li, CH,et al. Development of expressed sequence tags from the bay scallop, Argopecten irradians irradians[J]. MARINE BIOTECHNOLOGY,2006,8(2):161-169.
APA Song, LS.,Xu, W.,Li, CH.,Li, HL.,Wu, LT.,...&Guo, XM.(2006).Development of expressed sequence tags from the bay scallop, Argopecten irradians irradians.MARINE BIOTECHNOLOGY,8(2),161-169.
MLA Song, LS,et al."Development of expressed sequence tags from the bay scallop, Argopecten irradians irradians".MARINE BIOTECHNOLOGY 8.2(2006):161-169.
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