Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Biochemical and electron microscopic characterization of the F1F0 ATP Synthase from the hyperthermophilic eubacterium Aquifex aeolicus | |
Peng, Guohong; Bostina, Mihnea; Radermacher, Michael; Rais, Isam; Karas, Michael; Michel, Hartmut | |
2006-10-30 | |
发表期刊 | FEBS LETTERS |
ISSN | 0014-5793 |
卷号 | 580期号:25页码:5934-5940 |
文章类型 | Article |
摘要 | The F1F0 ATP synthase has been purified from the hyperthermophilic eubacterium Aquifex aeolicus and characterized. Its subunits have been identified by MALDI-mass spectrometry through peptide mass fingerprinting and MS/MS. It contains the canonical subunits alpha, beta, gamma, delta and epsilon of F-1 and subunits a and c of F-0. Two versions of the b subunit were found, which show a low sequence homology to each other. Most likely they form a heterodimer. An electron microscopic single particle analysis revealed clear structural details, including two stalks connecting F-1 and F-0. In several orientations the central stalk appears to be tilted and/or kinked. It is unclear whether there is a direct connection between the peripheral stalk and the 6 subunit. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.; The F1F0 ATP synthase has been purified from the hyperthermophilic eubacterium Aquifex aeolicus and characterized. Its subunits have been identified by MALDI-mass spectrometry through peptide mass fingerprinting and MS/MS. It contains the canonical subunits alpha, beta, gamma, delta and epsilon of F-1 and subunits a and c of F-0. Two versions of the b subunit were found, which show a low sequence homology to each other. Most likely they form a heterodimer. An electron microscopic single particle analysis revealed clear structural details, including two stalks connecting F-1 and F-0. In several orientations the central stalk appears to be tilted and/or kinked. It is unclear whether there is a direct connection between the peripheral stalk and the 6 subunit. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. |
关键词 | F1f0 Atpase b Subunit Mass Spectrometric Identification Electron Microscopic Single Particle Analysis Aquifex Aeolicus |
学科领域 | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
DOI | 10.1016/j.febslet.2006.09.062 |
URL | 查看原文 |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000241707100021 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/5898 |
专题 | 实验海洋生物学重点实验室 |
作者单位 | 1.Max Planck Inst Biophys, D-60438 Frankfurt, Germany 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 3.Univ Vermont, Dept Physiol & Mol Biophys, Burlington, VT 05405 USA 4.Univ Frankfurt, Inst Pharmaceut Chem, D-60439 Frankfurt, Germany |
推荐引用方式 GB/T 7714 | Peng, Guohong,Bostina, Mihnea,Radermacher, Michael,et al. Biochemical and electron microscopic characterization of the F1F0 ATP Synthase from the hyperthermophilic eubacterium Aquifex aeolicus[J]. FEBS LETTERS,2006,580(25):5934-5940. |
APA | Peng, Guohong,Bostina, Mihnea,Radermacher, Michael,Rais, Isam,Karas, Michael,&Michel, Hartmut.(2006).Biochemical and electron microscopic characterization of the F1F0 ATP Synthase from the hyperthermophilic eubacterium Aquifex aeolicus.FEBS LETTERS,580(25),5934-5940. |
MLA | Peng, Guohong,et al."Biochemical and electron microscopic characterization of the F1F0 ATP Synthase from the hyperthermophilic eubacterium Aquifex aeolicus".FEBS LETTERS 580.25(2006):5934-5940. |
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