Method for large-scale isolation and purification of R-phycoerythrin from red alga Polysiphonia urceolata Grev

doi:10.1016/j.pep.2006.02.001

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	Method for large-scale isolation and purification of R-phycoerythrin from red alga Polysiphonia urceolata Grev
	Niu, Jian-Feng ; Wang, Guang-Ce ; Tseng, Cheng-Kui
	2006-09-01
发表期刊	PROTEIN EXPRESSION AND PURIFICATION
ISSN	1046-5928
卷号	49 期号:1 页码:23-31
文章类型	Article
摘要	R-phycoerythrin was isolated and purified from a red alga, Polysiphonia urceolata Grev, using Streamline column combined with ion-exchange chromatography or hydroxyapatite chromatography. The purity of R-phycoerythrin isolated by Streamline column was up to 1.66 and the yield of R-phycoerythrin could be as high as 0.68 mg/g frozen P. urceolata. All the eluates from Streamline column were divided into two equivalent parts, respectively. One part was pumped into the ion-exchange column loaded with Q-Sepharose and the other was applied to the adsorption column loaded with hydroxyapatite. The purities of R-phycoerythrin purified using these two methods were both up to 3.26, more than 3.2 the commonly accepted criterion. The yield of purified R-phycoerythrin from the ion-exchange chromatography was 0.40 mg/g frozen P. urceolata and that from the hydroxyapatite chromatography could reach 0.34 mg/g frozen P. urceolata. The purified protein had three absorption peaks at 498, 535, and 565 nm and displayed a fluorescence maximum at 580 nm, which was consistent with the typical spectrum of R-phycoerythrin. The purified R-PE was also identified with electrophoresis. Only one single protein band appeared on native-PAGE with silver staining. SDS-PAGE demonstrated the presence of one 20 kDa major subunit, and one low intensity band corresponding to 33 kDa subunit. The results indicate that using the expanded bed adsorption combined with ion-exchange chromatography or hydroxyapatite chromatography, R-phycoerythrin can be purified from frozen P. urceolata on large scale. (c) 2006 Elsevier Inc. All rights reserved.; R-phycoerythrin was isolated and purified from a red alga, Polysiphonia urceolata Grev, using Streamline column combined with ion-exchange chromatography or hydroxyapatite chromatography. The purity of R-phycoerythrin isolated by Streamline column was up to 1.66 and the yield of R-phycoerythrin could be as high as 0.68 mg/g frozen P. urceolata. All the eluates from Streamline column were divided into two equivalent parts, respectively. One part was pumped into the ion-exchange column loaded with Q-Sepharose and the other was applied to the adsorption column loaded with hydroxyapatite. The purities of R-phycoerythrin purified using these two methods were both up to 3.26, more than 3.2 the commonly accepted criterion. The yield of purified R-phycoerythrin from the ion-exchange chromatography was 0.40 mg/g frozen P. urceolata and that from the hydroxyapatite chromatography could reach 0.34 mg/g frozen P. urceolata. The purified protein had three absorption peaks at 498, 535, and 565 nm and displayed a fluorescence maximum at 580 nm, which was consistent with the typical spectrum of R-phycoerythrin. The purified R-PE was also identified with electrophoresis. Only one single protein band appeared on native-PAGE with silver staining. SDS-PAGE demonstrated the presence of one 20 kDa major subunit, and one low intensity band corresponding to 33 kDa subunit. The results indicate that using the expanded bed adsorption combined with ion-exchange chromatography or hydroxyapatite chromatography, R-phycoerythrin can be purified from frozen P. urceolata on large scale. (c) 2006 Elsevier Inc. All rights reserved.
关键词	Polysiphonia Urceolata Grev R-phycoerythrin Purification Streamline Column Ion-exchange Chromatography Hydroxyapatite Chromatography
学科领域	Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
DOI	10.1016/j.pep.2006.02.001
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000240580500004
引用统计	被引频次：81[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5880
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Niu, Jian-Feng,Wang, Guang-Ce,Tseng, Cheng-Kui. Method for large-scale isolation and purification of R-phycoerythrin from red alga Polysiphonia urceolata Grev[J]. PROTEIN EXPRESSION AND PURIFICATION,2006,49(1):23-31.
APA	Niu, Jian-Feng,Wang, Guang-Ce,&Tseng, Cheng-Kui.(2006).Method for large-scale isolation and purification of R-phycoerythrin from red alga Polysiphonia urceolata Grev.PROTEIN EXPRESSION AND PURIFICATION,49(1),23-31.
MLA	Niu, Jian-Feng,et al."Method for large-scale isolation and purification of R-phycoerythrin from red alga Polysiphonia urceolata Grev".PROTEIN EXPRESSION AND PURIFICATION 49.1(2006):23-31.

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