Effects of infection of EGH-expressing Escherichia coli on haemocytes in Ciona intestinalis

doi:10.1016/j.jembe.2005.11.022

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	Effects of infection of EGH-expressing Escherichia coli on haemocytes in Ciona intestinalis
	Liu, LP ; Wu, CG ; Cben, TY ; Zhang, XJ; Li, FH ; Luo, W ; Xiang, JH
	2006-05-16
发表期刊	JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY
ISSN	0022-0981
卷号	332 期号:2 页码:121-134
文章类型	Article
摘要	The effects of infection of EGFP-expressing Escherichia coli on the haemocytes of the ascidian Ciona intestinalis were investigated. The results showed that THC of the infected individuals changed significantly. Hyaline amoebocytes phagocytosed E. coli in 5 min and excreted lysosome particles that attached to the surface of the bacteria. Granular amoebocytes released lots of particles for Immoral immunity while stem-cell-like haemocytes remained intact. With the increase in THC, the stem-cell-like haemocytes showed division and proliferation. A small portion of hyaline amoebocytes was at early apoptosis stage I h after infection and typical apoptosis bodies emerged in granular amoebocytes. A few of the infected haemocytes showed DNA damage using SCGE assay. Flow cytometry analysis revealed an obvious apoptosis peak in infected haemocytes. In conclusion, apoptosis was found to be an important immune response of ascidian haemocytes response to bacterial infection. To our best knowledge, this is the first report of the occurrence of apoptosis of haemocytes in ascidians. (c) 2005 Elsevier B.V. All rights reserved.; The effects of infection of EGFP-expressing Escherichia coli on the haemocytes of the ascidian Ciona intestinalis were investigated. The results showed that THC of the infected individuals changed significantly. Hyaline amoebocytes phagocytosed E. coli in 5 min and excreted lysosome particles that attached to the surface of the bacteria. Granular amoebocytes released lots of particles for Immoral immunity while stem-cell-like haemocytes remained intact. With the increase in THC, the stem-cell-like haemocytes showed division and proliferation. A small portion of hyaline amoebocytes was at early apoptosis stage I h after infection and typical apoptosis bodies emerged in granular amoebocytes. A few of the infected haemocytes showed DNA damage using SCGE assay. Flow cytometry analysis revealed an obvious apoptosis peak in infected haemocytes. In conclusion, apoptosis was found to be an important immune response of ascidian haemocytes response to bacterial infection. To our best knowledge, this is the first report of the occurrence of apoptosis of haemocytes in ascidians. (c) 2005 Elsevier B.V. All rights reserved.
关键词	Apoptosis Cellular Response Ciona Intestinalis Egfp-E.prE.sing E. Coli Haemocytes Infection
学科领域	Ecology ; Marine & Freshwater Biology
DOI	10.1016/j.jembe.2005.11.022
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000237767100001
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5868
专题	实验海洋生物学重点实验室海洋生态与环境科学重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Shanghai Fisheries Univ, Coll Aqua Life Sci & Technol, Shanghai 200090, Peoples R China 3.Chinese Acad Sci, Key Lab Marine Ecol & Environm Sci, Inst Oceanol, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Liu, LP,Wu, CG,Cben, TY,et al. Effects of infection of EGH-expressing Escherichia coli on haemocytes in Ciona intestinalis[J]. JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY,2006,332(2):121-134.
APA	Liu, LP.,Wu, CG.,Cben, TY.,Zhang, XJ.,Li, FH.,...&Xiang, JH.(2006).Effects of infection of EGH-expressing Escherichia coli on haemocytes in Ciona intestinalis.JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY,332(2),121-134.
MLA	Liu, LP,et al."Effects of infection of EGH-expressing Escherichia coli on haemocytes in Ciona intestinalis".JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY 332.2(2006):121-134.

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