Rapid optimization of process conditions for cultivation of transgenic Laminaria japonica gametophyte cells in a stirred-tank bioreactor

doi:10.1016/j.cej.2006.05.004

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	Rapid optimization of process conditions for cultivation of transgenic Laminaria japonica gametophyte cells in a stirred-tank bioreactor
	Gao, Jiangtao ; Qin, Song ; Zhang, Yicheng
	2006-09-01
Source Publication	CHEMICAL ENGINEERING JOURNAL
ISSN	1385-8947
Volume	122 Issue:1-2 Pages:11-14
Subtype	Article
Abstract	Batch cultivation for transgenic kelp gametophyte cells was investigated in an online controlled 5 L stirred-tank photo-bioreactor to rapidly optimize the process conditions by monitoring the rate of increase of pH. The transgenic kelp gametophytes with heterologous gene encoding hepatitis B surface antigen (HBsAg) could rapidly grow in the bioreactor. Optimal temperature and agitation rate for bioreactor cultivation of gametophytes were 15 degrees C and 200 rpm. Optimal incident light intensities depended on the initial cell densities. (c) 2006 Elsevier B.V. All fights reserved.; Batch cultivation for transgenic kelp gametophyte cells was investigated in an online controlled 5 L stirred-tank photo-bioreactor to rapidly optimize the process conditions by monitoring the rate of increase of pH. The transgenic kelp gametophytes with heterologous gene encoding hepatitis B surface antigen (HBsAg) could rapidly grow in the bioreactor. Optimal temperature and agitation rate for bioreactor cultivation of gametophytes were 15 degrees C and 200 rpm. Optimal incident light intensities depended on the initial cell densities. (c) 2006 Elsevier B.V. All fights reserved.
Keyword	Gametophyte Cells Hbsag Laminaria Japonica Ph Process Conditions Stirred-tank Bioreactor
Subject Area	Engineering, Environmental ; Engineering, Chemical
DOI	10.1016/j.cej.2006.05.004
URL	查看原文
Indexed By	SCI
Language	英语
WOS ID	WOS:000240586000002
Citation statistics	Cited Times:4[WOS] [WOS Record] [Related Records in WOS]
Document Type	期刊论文
Identifier	http://ir.qdio.ac.cn/handle/337002/5830
Collection	实验海洋生物学重点实验室
Affiliation	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation GB/T 7714	Gao, Jiangtao,Qin, Song,Zhang, Yicheng. Rapid optimization of process conditions for cultivation of transgenic Laminaria japonica gametophyte cells in a stirred-tank bioreactor[J]. CHEMICAL ENGINEERING JOURNAL,2006,122(1-2):11-14.
APA	Gao, Jiangtao,Qin, Song,&Zhang, Yicheng.(2006).Rapid optimization of process conditions for cultivation of transgenic Laminaria japonica gametophyte cells in a stirred-tank bioreactor.CHEMICAL ENGINEERING JOURNAL,122(1-2),11-14.
MLA	Gao, Jiangtao,et al."Rapid optimization of process conditions for cultivation of transgenic Laminaria japonica gametophyte cells in a stirred-tank bioreactor".CHEMICAL ENGINEERING JOURNAL 122.1-2(2006):11-14.

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