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Rapid optimization of process conditions for cultivation of transgenic Laminaria japonica gametophyte cells in a stirred-tank bioreactor
Gao, Jiangtao; Qin, Song; Zhang, Yicheng
2006-09-01
Source PublicationCHEMICAL ENGINEERING JOURNAL
ISSN1385-8947
Volume122Issue:1-2Pages:11-14
SubtypeArticle
AbstractBatch cultivation for transgenic kelp gametophyte cells was investigated in an online controlled 5 L stirred-tank photo-bioreactor to rapidly optimize the process conditions by monitoring the rate of increase of pH. The transgenic kelp gametophytes with heterologous gene encoding hepatitis B surface antigen (HBsAg) could rapidly grow in the bioreactor. Optimal temperature and agitation rate for bioreactor cultivation of gametophytes were 15 degrees C and 200 rpm. Optimal incident light intensities depended on the initial cell densities. (c) 2006 Elsevier B.V. All fights reserved.; Batch cultivation for transgenic kelp gametophyte cells was investigated in an online controlled 5 L stirred-tank photo-bioreactor to rapidly optimize the process conditions by monitoring the rate of increase of pH. The transgenic kelp gametophytes with heterologous gene encoding hepatitis B surface antigen (HBsAg) could rapidly grow in the bioreactor. Optimal temperature and agitation rate for bioreactor cultivation of gametophytes were 15 degrees C and 200 rpm. Optimal incident light intensities depended on the initial cell densities. (c) 2006 Elsevier B.V. All fights reserved.
KeywordGametophyte Cells Hbsag Laminaria Japonica Ph Process Conditions Stirred-tank Bioreactor
Subject AreaEngineering, Environmental ; Engineering, Chemical
DOI10.1016/j.cej.2006.05.004
URL查看原文
Indexed BySCI
Language英语
WOS IDWOS:000240586000002
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/5830
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Gao, Jiangtao,Qin, Song,Zhang, Yicheng. Rapid optimization of process conditions for cultivation of transgenic Laminaria japonica gametophyte cells in a stirred-tank bioreactor[J]. CHEMICAL ENGINEERING JOURNAL,2006,122(1-2):11-14.
APA Gao, Jiangtao,Qin, Song,&Zhang, Yicheng.(2006).Rapid optimization of process conditions for cultivation of transgenic Laminaria japonica gametophyte cells in a stirred-tank bioreactor.CHEMICAL ENGINEERING JOURNAL,122(1-2),11-14.
MLA Gao, Jiangtao,et al."Rapid optimization of process conditions for cultivation of transgenic Laminaria japonica gametophyte cells in a stirred-tank bioreactor".CHEMICAL ENGINEERING JOURNAL 122.1-2(2006):11-14.
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