Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Rapid optimization of process conditions for cultivation of transgenic Laminaria japonica gametophyte cells in a stirred-tank bioreactor | |
Gao, Jiangtao; Qin, Song; Zhang, Yicheng | |
2006-09-01 | |
Source Publication | CHEMICAL ENGINEERING JOURNAL
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ISSN | 1385-8947 |
Volume | 122Issue:1-2Pages:11-14 |
Subtype | Article |
Abstract | Batch cultivation for transgenic kelp gametophyte cells was investigated in an online controlled 5 L stirred-tank photo-bioreactor to rapidly optimize the process conditions by monitoring the rate of increase of pH. The transgenic kelp gametophytes with heterologous gene encoding hepatitis B surface antigen (HBsAg) could rapidly grow in the bioreactor. Optimal temperature and agitation rate for bioreactor cultivation of gametophytes were 15 degrees C and 200 rpm. Optimal incident light intensities depended on the initial cell densities. (c) 2006 Elsevier B.V. All fights reserved.; Batch cultivation for transgenic kelp gametophyte cells was investigated in an online controlled 5 L stirred-tank photo-bioreactor to rapidly optimize the process conditions by monitoring the rate of increase of pH. The transgenic kelp gametophytes with heterologous gene encoding hepatitis B surface antigen (HBsAg) could rapidly grow in the bioreactor. Optimal temperature and agitation rate for bioreactor cultivation of gametophytes were 15 degrees C and 200 rpm. Optimal incident light intensities depended on the initial cell densities. (c) 2006 Elsevier B.V. All fights reserved. |
Keyword | Gametophyte Cells Hbsag Laminaria Japonica Ph Process Conditions Stirred-tank Bioreactor |
Subject Area | Engineering, Environmental ; Engineering, Chemical |
DOI | 10.1016/j.cej.2006.05.004 |
URL | 查看原文 |
Indexed By | SCI |
Language | 英语 |
WOS ID | WOS:000240586000002 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.qdio.ac.cn/handle/337002/5830 |
Collection | 实验海洋生物学重点实验室 |
Affiliation | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
Recommended Citation GB/T 7714 | Gao, Jiangtao,Qin, Song,Zhang, Yicheng. Rapid optimization of process conditions for cultivation of transgenic Laminaria japonica gametophyte cells in a stirred-tank bioreactor[J]. CHEMICAL ENGINEERING JOURNAL,2006,122(1-2):11-14. |
APA | Gao, Jiangtao,Qin, Song,&Zhang, Yicheng.(2006).Rapid optimization of process conditions for cultivation of transgenic Laminaria japonica gametophyte cells in a stirred-tank bioreactor.CHEMICAL ENGINEERING JOURNAL,122(1-2),11-14. |
MLA | Gao, Jiangtao,et al."Rapid optimization of process conditions for cultivation of transgenic Laminaria japonica gametophyte cells in a stirred-tank bioreactor".CHEMICAL ENGINEERING JOURNAL 122.1-2(2006):11-14. |
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