IOCAS-IR  > 实验海洋生物学重点实验室
Expression of Scophthalmus maximus CD83 correlates with bacterial infection and antigen stimulation
Hu, Yong-hua; Zhang, Min; Sun, Li
2010-10-01
发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
卷号29期号:4页码:608-614
文章类型Article
摘要CD83 is a transmembrane glycoprotein of the immunoglobulin (Ig) superfamily and a surface marker for fully matured dendritic cells (DCs) in humans and mice. In teleosts, DC-like cells and their molecular markers are largely unknown. In this report, we described the identification and expressional analysis of a CD83 homologue, SmCD83, from turbot Scophthalmus maximus. The open reading frame of SmCD83 is 639 bp, which is preceded by a S'-untranslated region (UTR) of 87 bp and followed by a 3'-UTR of 1111 bp. The SmCD83 gene is 4716 bp in length, which contains five exons and four introns. The deduced amino acid sequence of SmCD83 shares 40-50% overall identities with the CD83 of several fish species. Like typical CD83, SmCD83 possesses an Ig-like extracellular domain, a transmembrane domain, and a cytoplasmic domain. The conserved disulfide bond-forming cysteine residues and the N-linked glycosylation sites that are preserved in CD83 are also found in SmCD83. Expressional analysis showed that constitutive expression of SmCD83 was high in gill, blood, spleen, muscle, and kidney and low in heart and liver. Bacterial infection and poly(I:C) treatment enhanced SmCD83 expression in kidney in time-dependent manners. Likewise, bacterial challenge caused significant induction of SmCD83 expression in cultured macrophages. Vaccination of turbot with a bacterin and a purified recombinant subunit vaccine-induced significant SmCD83 expression during the first week following vaccination. These results demonstrate that SmCD83 expression correlates with microbial challenge and antigen stimulation, which suggests the possibility that there may exist in turbot DC-like antigen-presenting cells that express SmCD83 upon activation by antigen uptake. In addition, these results also suggest that SmCD83 may serve as a marker for activated macrophages in turbot. (C) 2010 Elsevier Ltd. All rights reserved.; CD83 is a transmembrane glycoprotein of the immunoglobulin (Ig) superfamily and a surface marker for fully matured dendritic cells (DCs) in humans and mice. In teleosts, DC-like cells and their molecular markers are largely unknown. In this report, we described the identification and expressional analysis of a CD83 homologue, SmCD83, from turbot Scophthalmus maximus. The open reading frame of SmCD83 is 639 bp, which is preceded by a S'-untranslated region (UTR) of 87 bp and followed by a 3'-UTR of 1111 bp. The SmCD83 gene is 4716 bp in length, which contains five exons and four introns. The deduced amino acid sequence of SmCD83 shares 40-50% overall identities with the CD83 of several fish species. Like typical CD83, SmCD83 possesses an Ig-like extracellular domain, a transmembrane domain, and a cytoplasmic domain. The conserved disulfide bond-forming cysteine residues and the N-linked glycosylation sites that are preserved in CD83 are also found in SmCD83. Expressional analysis showed that constitutive expression of SmCD83 was high in gill, blood, spleen, muscle, and kidney and low in heart and liver. Bacterial infection and poly(I:C) treatment enhanced SmCD83 expression in kidney in time-dependent manners. Likewise, bacterial challenge caused significant induction of SmCD83 expression in cultured macrophages. Vaccination of turbot with a bacterin and a purified recombinant subunit vaccine-induced significant SmCD83 expression during the first week following vaccination. These results demonstrate that SmCD83 expression correlates with microbial challenge and antigen stimulation, which suggests the possibility that there may exist in turbot DC-like antigen-presenting cells that express SmCD83 upon activation by antigen uptake. In addition, these results also suggest that SmCD83 may serve as a marker for activated macrophages in turbot. (C) 2010 Elsevier Ltd. All rights reserved.
关键词Cd83 Scophthalmus Maximus Dendritic Cell Antigen-presenting
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI10.1016/j.fsi.2010.06.014
URL查看原文
收录类别SCI
语种英语
WOS记录号WOS:000281500500009
引用统计
被引频次:21[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/5826
专题实验海洋生物学重点实验室
作者单位Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
第一作者单位中国科学院海洋研究所
推荐引用方式
GB/T 7714
Hu, Yong-hua,Zhang, Min,Sun, Li. Expression of Scophthalmus maximus CD83 correlates with bacterial infection and antigen stimulation[J]. FISH & SHELLFISH IMMUNOLOGY,2010,29(4):608-614.
APA Hu, Yong-hua,Zhang, Min,&Sun, Li.(2010).Expression of Scophthalmus maximus CD83 correlates with bacterial infection and antigen stimulation.FISH & SHELLFISH IMMUNOLOGY,29(4),608-614.
MLA Hu, Yong-hua,et al."Expression of Scophthalmus maximus CD83 correlates with bacterial infection and antigen stimulation".FISH & SHELLFISH IMMUNOLOGY 29.4(2010):608-614.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Expression of Scopht(1395KB) 限制开放--浏览
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Hu, Yong-hua]的文章
[Zhang, Min]的文章
[Sun, Li]的文章
百度学术
百度学术中相似的文章
[Hu, Yong-hua]的文章
[Zhang, Min]的文章
[Sun, Li]的文章
必应学术
必应学术中相似的文章
[Hu, Yong-hua]的文章
[Zhang, Min]的文章
[Sun, Li]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Expression of Scophthalmus maximus CD83 correlates with bacterial infection and antigen stimulation.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。