Molecular cloning, characterization and expression of a serine protease with clip-domain homologue from scallop Chlamys farreri

doi:10.1016/j.fsi.2006.08.002

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	Molecular cloning, characterization and expression of a serine protease with clip-domain homologue from scallop Chlamys farreri
	Zhu, Ling ; Song, Linsheng ; Zhao, Jiangmin ; Xu, Wei ; Chang, Yaqing
	2007-05-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	22 期号:5 页码:556-566
文章类型	Article
摘要	Serine proteases play critical roles in a variety of invertebrate immune defense responses, including hemolymph coagulation, antimicrobial peptide synthesis, and melanization. The first mollusk serine protease with clip-domain (designated CFSP1) cDNA was obtained from the scallop Chlamys farreri challenged with Vibrio anguillarum by randomly sequencing a whole tissue cDNA library and rapid amplification of cDNA ends (RACE). The full-length cDNA of the C. farreri serine protease was 1211 bp, consisting of a 5-terminal untranslated region (UTR) of 72 bp, a 3'-terminal UTR of 77 bp with a canonical polyadenylation signal sequence AATAAA and a poly (A) tail, and an open reading frame of 1062 bp. The CFSP1 cDNA encoded a polypeptide of 354 amino acids with a putative signal peptide of 19 amino acids and a mature protein of 335 amino acids. The deduced amino acid sequence of CFSP1 contained an amino-terminal clip domain, a low complexity region, and a carboxyl-terminal serine protease domain. CFSP1 mRNA was mainly expressed constitutively in the hemocytes and was up-regulated and increased 2.9- and 1.9-fold at 16 h after injury and injection of bacteria. (c) 2006 Elsevier Ltd. All rights reserved.; Serine proteases play critical roles in a variety of invertebrate immune defense responses, including hemolymph coagulation, antimicrobial peptide synthesis, and melanization. The first mollusk serine protease with clip-domain (designated CFSP1) cDNA was obtained from the scallop Chlamys farreri challenged with Vibrio anguillarum by randomly sequencing a whole tissue cDNA library and rapid amplification of cDNA ends (RACE). The full-length cDNA of the C. farreri serine protease was 1211 bp, consisting of a 5-terminal untranslated region (UTR) of 72 bp, a 3'-terminal UTR of 77 bp with a canonical polyadenylation signal sequence AATAAA and a poly (A) tail, and an open reading frame of 1062 bp. The CFSP1 cDNA encoded a polypeptide of 354 amino acids with a putative signal peptide of 19 amino acids and a mature protein of 335 amino acids. The deduced amino acid sequence of CFSP1 contained an amino-terminal clip domain, a low complexity region, and a carboxyl-terminal serine protease domain. CFSP1 mRNA was mainly expressed constitutively in the hemocytes and was up-regulated and increased 2.9- and 1.9-fold at 16 h after injury and injection of bacteria. (c) 2006 Elsevier Ltd. All rights reserved.
关键词	Serine Protease Clip Domain Chlamys Farreri Tissue Distribution Mrna Expression Injury Healing Immune Response
学科领域	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI	10.1016/j.fsi.2006.08.002
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000245208300011
引用统计	被引频次：17[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5780
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Dalian Fisheries Coll, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Ling,Song, Linsheng,Zhao, Jiangmin,et al. Molecular cloning, characterization and expression of a serine protease with clip-domain homologue from scallop Chlamys farreri[J]. FISH & SHELLFISH IMMUNOLOGY,2007,22(5):556-566.
APA	Zhu, Ling,Song, Linsheng,Zhao, Jiangmin,Xu, Wei,&Chang, Yaqing.(2007).Molecular cloning, characterization and expression of a serine protease with clip-domain homologue from scallop Chlamys farreri.FISH & SHELLFISH IMMUNOLOGY,22(5),556-566.
MLA	Zhu, Ling,et al."Molecular cloning, characterization and expression of a serine protease with clip-domain homologue from scallop Chlamys farreri".FISH & SHELLFISH IMMUNOLOGY 22.5(2007):556-566.

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