The encysted dormant embryo proteome of Artemia sinica

doi:10.1007/s10126-007-9079-0

IOCAS-IR > 实验海洋生物学重点实验室

	The encysted dormant embryo proteome of Artemia sinica
	Zhou, Qian 1,2; Wu, Changgong 1; Dong, Bo 1; Liu, Fengqi 3; Xiang, Jianhai1
	2008-07-01
发表期刊	MARINE BIOTECHNOLOGY
ISSN	1436-2228
卷号	10 期号:4 页码:438-446
文章类型	Article
摘要	The possibility of the brine shrimp Artemia to produce dormant embryo (cysts) in diapause is a key feature in its life history. In the present study, we obtained a proteomic reference map for the diapause embryo of Artemia sinica using two-dimensional gel electrophoresis with a pH range of 4-7 and a molecular weight range of 10-100 kDa. Approximately 233 proteins were detected, and 60 of them were analyzed by capillary liquid chromatography tandem mass spectrometry (LC-MS/MS). Of these, 39 spots representing 33 unique proteins were identified, which are categorized into functional groups, including cell defense, cell structure, metabolism, protein synthesis, proteolysis, and other processes. This reference map will contribute toward understanding the state of the diapause embryo and lay the basis and serve as a useful tool for further profound studies in the proteomics of Artemia at different developmental stages and physiological conditions.; The possibility of the brine shrimp Artemia to produce dormant embryo (cysts) in diapause is a key feature in its life history. In the present study, we obtained a proteomic reference map for the diapause embryo of Artemia sinica using two-dimensional gel electrophoresis with a pH range of 4-7 and a molecular weight range of 10-100 kDa. Approximately 233 proteins were detected, and 60 of them were analyzed by capillary liquid chromatography tandem mass spectrometry (LC-MS/MS). Of these, 39 spots representing 33 unique proteins were identified, which are categorized into functional groups, including cell defense, cell structure, metabolism, protein synthesis, proteolysis, and other processes. This reference map will contribute toward understanding the state of the diapause embryo and lay the basis and serve as a useful tool for further profound studies in the proteomics of Artemia at different developmental stages and physiological conditions.
关键词	Proteome Diapause Embryo 2d-e Mass Spectrometry Artemia Sinica
学科领域	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
DOI	10.1007/s10126-007-9079-0
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000256434600011
引用统计	被引频次：26[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5768
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Nankai Univ, Coll Life Sci, Tianjin 300071, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhou, Qian,Wu, Changgong,Dong, Bo,et al. The encysted dormant embryo proteome of Artemia sinica[J]. MARINE BIOTECHNOLOGY,2008,10(4):438-446.
APA	Zhou, Qian,Wu, Changgong,Dong, Bo,Liu, Fengqi,&Xiang, Jianhai.(2008).The encysted dormant embryo proteome of Artemia sinica.MARINE BIOTECHNOLOGY,10(4),438-446.
MLA	Zhou, Qian,et al."The encysted dormant embryo proteome of Artemia sinica".MARINE BIOTECHNOLOGY 10.4(2008):438-446.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
zhou-The Encysted Do（215KB）			限制开放	--	浏览