EscC is a chaperone for the Edwardsiella tarda type III secretion system putative translocon components EseB and EseD

doi:10.1099/mic.0.2006/004592-0

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	EscC is a chaperone for the Edwardsiella tarda type III secretion system putative translocon components EseB and EseD
	Zheng, Jun ; Li, Nan ; Tan, Yuen Peng ; Sivaraman, J.; Mok, Yu-Keung ; Mo, Zhao Lan ; Leung, Ka Yin
	2007-06-01
发表期刊	MICROBIOLOGY-SGM
ISSN	1350-0872
卷号	153 期号:Part 6 页码:1953-1962
文章类型	Article
摘要	Edwardsiella tarda is a Gram-negative enteric pathogen that causes disease in both humans and animals. Recently, a type III secretion system (T3SS) has been found to contribute to Ed. tarda pathogenesis. EseB, EseC and EseD were shown to be secreted by the T3SS and to be the major components of the extracellular proteins (ECPs). Based on sequence similarity, they have been proposed to function as the 'translocon' of the T3SS needle structure. In this study, it was shown that EseB, EseC and EseD formed a protein complex after secretion, which is consistent with their possible roles as translocon components. The secretion of EseB and EseD was dependent on EscC (previously named Orf2). EscC has the characteristics of a chaperone; it is a small protein (13 kDa), located next to the translocators in the T3SS gene cluster, and has a coiled-coil structure at the N-terminal region as predicted by COILS. An in-frame deletion of escC abolished the secretion of EseB and EseD, and complementation of Delta escC restored the export of EseB and EseD into the culture supernatant. Further studies showed that EscC is not a secreted protein and is located on the membrane and in the cytoplasm. Mutation of escC did not affect the transcription of eseB but reduced the amount of EseB as measured by using an EseB-LacZ fusion protein in Ed. tarda. Co-purification studies demonstrated that EscC formed complexes with EseB and EseD. The results suggest that EscC functions as a T3SS chaperone for the putative translocon components EseB and EseD in Ed. tarda.; Edwardsiella tarda is a Gram-negative enteric pathogen that causes disease in both humans and animals. Recently, a type III secretion system (T3SS) has been found to contribute to Ed. tarda pathogenesis. EseB, EseC and EseD were shown to be secreted by the T3SS and to be the major components of the extracellular proteins (ECPs). Based on sequence similarity, they have been proposed to function as the 'translocon' of the T3SS needle structure. In this study, it was shown that EseB, EseC and EseD formed a protein complex after secretion, which is consistent with their possible roles as translocon components. The secretion of EseB and EseD was dependent on EscC (previously named Orf2). EscC has the characteristics of a chaperone; it is a small protein (13 kDa), located next to the translocators in the T3SS gene cluster, and has a coiled-coil structure at the N-terminal region as predicted by COILS. An in-frame deletion of escC abolished the secretion of EseB and EseD, and complementation of Delta escC restored the export of EseB and EseD into the culture supernatant. Further studies showed that EscC is not a secreted protein and is located on the membrane and in the cytoplasm. Mutation of escC did not affect the transcription of eseB but reduced the amount of EseB as measured by using an EseB-LacZ fusion protein in Ed. tarda. Co-purification studies demonstrated that EscC formed complexes with EseB and EseD. The results suggest that EscC functions as a T3SS chaperone for the putative translocon components EseB and EseD in Ed. tarda.
关键词	Enteropathogenic Escherichia-coli Salmonella Pathogenicity Island-2 Virulence Genes Proteins Bacteria Export Espb Fish Identification Pathogenesis
学科领域	Microbiology
DOI	10.1099/mic.0.2006/004592-0
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000247304900027
引用统计	被引频次：29[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5766
专题	实验海洋生物学重点实验室
作者单位	1.Natl Univ Singapore, Fac Sci, Dept Biol Sci, Singapore 117543, Singapore 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Zheng, Jun,Li, Nan,Tan, Yuen Peng,et al. EscC is a chaperone for the Edwardsiella tarda type III secretion system putative translocon components EseB and EseD[J]. MICROBIOLOGY-SGM,2007,153(Part 6):1953-1962.
APA	Zheng, Jun.,Li, Nan.,Tan, Yuen Peng.,Sivaraman, J..,Mok, Yu-Keung.,...&Leung, Ka Yin.(2007).EscC is a chaperone for the Edwardsiella tarda type III secretion system putative translocon components EseB and EseD.MICROBIOLOGY-SGM,153(Part 6),1953-1962.
MLA	Zheng, Jun,et al."EscC is a chaperone for the Edwardsiella tarda type III secretion system putative translocon components EseB and EseD".MICROBIOLOGY-SGM 153.Part 6(2007):1953-1962.

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