Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recombinant expression of rt-PA gene (encoding Reteplase) in gametophytes of the seaweed Laminaria japonica (Laminariales, Phaeophyta) | |
Zhang YiChen1,2; Jiang Peng1; Gao JiangTao1,3; Liao JianMin4; Sun ShiJing4; Shen ZiLong4; Qin Song1 | |
2008-12-01 | |
发表期刊 | SCIENCE IN CHINA SERIES C-LIFE SCIENCES |
ISSN | 1006-9305 |
卷号 | 51期号:12页码:1116-1120 |
文章类型 | Article |
摘要 | The life cycle of seaweed Laminaria japonica involves a generation alternation between diploid sporophyte and haploid gametophte. The expression of foreign genes in sporophte has been proved. In this research, the recombinant expression in gametophyte was investigated by particle bombardment with the rt-PA gene encoding the recombinant human tissue-type plasminogen activator (Reteplase), which is a thrombolytic agent for acute myocardial infarction (AMI). Transgenic gametophytes were selected by their resistance to herbicide phosphiothricin (PPT), and proliferated in an established bubble column photo-bioreactor. According to the results from quantitative ELISA, Southern blotting, and fibrin agarose plate assay (FAPA) for bioactivity, it was showed that the rt-PA gene had been integrated into the genome of gametophytes of L. japonica, and the expression product showed the expected bioactivity, implying the proper post-transcript modification in haploid gametophyte.; The life cycle of seaweed Laminaria japonica involves a generation alternation between diploid sporophyte and haploid gametophte. The expression of foreign genes in sporophte has been proved. In this research, the recombinant expression in gametophyte was investigated by particle bombardment with the rt-PA gene encoding the recombinant human tissue-type plasminogen activator (Reteplase), which is a thrombolytic agent for acute myocardial infarction (AMI). Transgenic gametophytes were selected by their resistance to herbicide phosphiothricin (PPT), and proliferated in an established bubble column photo-bioreactor. According to the results from quantitative ELISA, Southern blotting, and fibrin agarose plate assay (FAPA) for bioactivity, it was showed that the rt-PA gene had been integrated into the genome of gametophytes of L. japonica, and the expression product showed the expected bioactivity, implying the proper post-transcript modification in haploid gametophyte. |
关键词 | Laminaria Japonica Gametophyte Genetic Engineering Reteplase Rt-pa Bioreactor |
学科领域 | Biology |
DOI | 10.1007/s11427-008-0143-4 |
URL | 查看原文 |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000261832500009 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/5744 |
专题 | 实验海洋生物学重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Tianjin Normal Univ, Coll Chem & Life Sci, Tianjin 300387, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 4.China Pharmaceut Univ, Ctr Biotechnol, Nanjing 210009, Peoples R China |
第一作者单位 | 中国科学院海洋研究所 |
推荐引用方式 GB/T 7714 | Zhang YiChen,Jiang Peng,Gao JiangTao,et al. Recombinant expression of rt-PA gene (encoding Reteplase) in gametophytes of the seaweed Laminaria japonica (Laminariales, Phaeophyta)[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2008,51(12):1116-1120. |
APA | Zhang YiChen.,Jiang Peng.,Gao JiangTao.,Liao JianMin.,Sun ShiJing.,...&Qin Song.(2008).Recombinant expression of rt-PA gene (encoding Reteplase) in gametophytes of the seaweed Laminaria japonica (Laminariales, Phaeophyta).SCIENCE IN CHINA SERIES C-LIFE SCIENCES,51(12),1116-1120. |
MLA | Zhang YiChen,et al."Recombinant expression of rt-PA gene (encoding Reteplase) in gametophytes of the seaweed Laminaria japonica (Laminariales, Phaeophyta)".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 51.12(2008):1116-1120. |
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