Molecular cloning, expression and characterization of a chitosanase from Microbacterium sp.

doi:10.1007/s10529-007-9373-y

IOCAS-IR > 实验海洋生物学重点实验室

	Molecular cloning, expression and characterization of a chitosanase from Microbacterium sp.
	Zhang, Jiquan ; Sun, Yuying
	2007-08-01
发表期刊	BIOTECHNOLOGY LETTERS
ISSN	0141-5492
卷号	29 期号:8 页码:1221-1225
文章类型	Article
摘要	A gene encoding a chitosanase (mschito) was cloned from Microbacterium, sp. OU01. The ORF consists of 801 bp which encoded a polypeptide of 266 amino acid residues. The deduced amino acid sequence shows 98% identity to that of the chitosanase reported in Pseudomonas sp. A-01. In addition, the fusion protein containing MSCHITO was expressed in E. coli and purified using Ni-NTA affinity chromatography. The purified rMSCHITO protein degraded the chitosan (the degree of deacetylation of 99%) and produced a mixture of chitooligosaccharides. The MSCHITO is thus an endo-chitosanase.; A gene encoding a chitosanase (mschito) was cloned from Microbacterium, sp. OU01. The ORF consists of 801 bp which encoded a polypeptide of 266 amino acid residues. The deduced amino acid sequence shows 98% identity to that of the chitosanase reported in Pseudomonas sp. A-01. In addition, the fusion protein containing MSCHITO was expressed in E. coli and purified using Ni-NTA affinity chromatography. The purified rMSCHITO protein degraded the chitosan (the degree of deacetylation of 99%) and produced a mixture of chitooligosaccharides. The MSCHITO is thus an endo-chitosanase.
关键词	Chitosanase Chitooligosaccharides Chitosan Cloning Microbacterium Sp.
学科领域	Biotechnology & Applied Microbiology
DOI	10.1007/s10529-007-9373-y
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000248224600012
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5738
专题	实验海洋生物学重点实验室
作者单位	1.Huaihai Inst Technol, Key Lab Marine Biotechnol Jiangsu Province, Lianyungang 222005, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Jiquan,Sun, Yuying. Molecular cloning, expression and characterization of a chitosanase from Microbacterium sp.[J]. BIOTECHNOLOGY LETTERS,2007,29(8):1221-1225.
APA	Zhang, Jiquan,&Sun, Yuying.(2007).Molecular cloning, expression and characterization of a chitosanase from Microbacterium sp..BIOTECHNOLOGY LETTERS,29(8),1221-1225.
MLA	Zhang, Jiquan,et al."Molecular cloning, expression and characterization of a chitosanase from Microbacterium sp.".BIOTECHNOLOGY LETTERS 29.8(2007):1221-1225.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
zhang-Molecular clon（211KB）			限制开放	--	浏览