Expression, purification, and characterization of recombinant Chinese shrimp crustin-like protein (CruFc) in Pichia pastoris

doi:10.1007/s10529-007-9317-6

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	Expression, purification, and characterization of recombinant Chinese shrimp crustin-like protein (CruFc) in Pichia pastoris
	Zhang, Jiquan; Li, Fuhua; Wang, Zaizhao; Xiang, Jianhai
	2007-05-01
发表期刊	BIOTECHNOLOGY LETTERS
ISSN	0141-5492
卷号	29 期号:5 页码:813-817
文章类型	Article
摘要	A crustin-like protein (CruFc) from Fenneropenaeus chinensis was expressed in Pichia pastoris and then purified to electrophoretic homogeneity on a Sephacryl S-100 column with a band corresponding to the expected one (13 kDa) shown by 15% SDS-PAGE. Western blot indicated that the rCruFc specifically reacted with polyclonal rabbit anti-Fenneropenaeus chinensis CruFc. Production in a 5 l bioreactor gave 237 mg rCruFc/l. Antimicrobial assay revealed that 4 mu M rCruFc inhibited growth of Staphylococcus aureus.; A crustin-like protein (CruFc) from Fenneropenaeus chinensis was expressed in Pichia pastoris and then purified to electrophoretic homogeneity on a Sephacryl S-100 column with a band corresponding to the expected one (13 kDa) shown by 15% SDS-PAGE. Western blot indicated that the rCruFc specifically reacted with polyclonal rabbit anti-Fenneropenaeus chinensis CruFc. Production in a 5 l bioreactor gave 237 mg rCruFc/l. Antimicrobial assay revealed that 4 mu M rCruFc inhibited growth of Staphylococcus aureus.
关键词	Crustin-like Protein Fenneropenaeus Chinensis Pichia Pastoris
学科领域	Biotechnology & Applied Microbiology
DOI	10.1007/s10529-007-9317-6
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000245519800020
引用统计	被引频次：27[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5734
专题	实验海洋生物学重点实验室
作者单位	Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Jiquan,Li, Fuhua,Wang, Zaizhao,et al. Expression, purification, and characterization of recombinant Chinese shrimp crustin-like protein (CruFc) in Pichia pastoris[J]. BIOTECHNOLOGY LETTERS,2007,29(5):813-817.
APA	Zhang, Jiquan,Li, Fuhua,Wang, Zaizhao,&Xiang, Jianhai.(2007).Expression, purification, and characterization of recombinant Chinese shrimp crustin-like protein (CruFc) in Pichia pastoris.BIOTECHNOLOGY LETTERS,29(5),813-817.
MLA	Zhang, Jiquan,et al."Expression, purification, and characterization of recombinant Chinese shrimp crustin-like protein (CruFc) in Pichia pastoris".BIOTECHNOLOGY LETTERS 29.5(2007):813-817.

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