Construction of bacterial artificial chromosome libraries for Zhikong Scallop Chlamys farreri

doi:10.1007/s00343-008-0215-0

IOCAS-IR > 实验海洋生物学重点实验室

	Construction of bacterial artificial chromosome libraries for Zhikong Scallop Chlamys farreri
	Zhang Yang 1,2,3; Zhang Xiaojun 1,3; Scheuring, Chantel E.3; Zhang Hongbin 3; Li Fuhua 1; Xiang Jianhai1
	2008-05-01
发表期刊	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN	0254-4059
卷号	26 期号:2 页码:215-218
文章类型	Article
摘要	Two Large-insert genomic bacterial artificial chromosome (BAC) libraries of Zhikong scallop Chlamys farreri were constructed to promote our genetic and genomic research. High-quality megabase-sized DNA was isolated from the adductor muscle of the scallop and partially digested by BamH I and Mbo I, respectively. The BamH I library consisted of 53 760 clones while the Mbo I library consisted of 7 680clones. Approximately 96 % of the clones in BamH I library contained nuclear DNA inserts in average size of 100 kb, providing a coverage of 5.3 haploid genome equivalents. Similarly, the Mbo I library with an average insert of 145 kb and no insert-empty clones, thus providing a genome coverage of 1.1 haploid genome equivalents.; Two Large-insert genomic bacterial artificial chromosome (BAC) libraries of Zhikong scallop Chlamys farreri were constructed to promote our genetic and genomic research. High-quality megabase-sized DNA was isolated from the adductor muscle of the scallop and partially digested by BamH I and Mbo I, respectively. The BamH I library consisted of 53 760 clones while the Mbo I library consisted of 7 680clones. Approximately 96 % of the clones in BamH I library contained nuclear DNA inserts in average size of 100 kb, providing a coverage of 5.3 haploid genome equivalents. Similarly, the Mbo I library with an average insert of 145 kb and no insert-empty clones, thus providing a genome coverage of 1.1 haploid genome equivalents.
关键词	Bac Library Zhikong Scallop Chlamys Farreri Genome
学科领域	Limnology ; Oceanography
DOI	10.1007/s00343-008-0215-0
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000256416000015
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5728
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhang Yang,Zhang Xiaojun,Scheuring, Chantel E.,et al. Construction of bacterial artificial chromosome libraries for Zhikong Scallop Chlamys farreri[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2008,26(2):215-218.
APA	Zhang Yang,Zhang Xiaojun,Scheuring, Chantel E.,Zhang Hongbin,Li Fuhua,&Xiang Jianhai.(2008).Construction of bacterial artificial chromosome libraries for Zhikong Scallop Chlamys farreri.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,26(2),215-218.
MLA	Zhang Yang,et al."Construction of bacterial artificial chromosome libraries for Zhikong Scallop Chlamys farreri".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 26.2(2008):215-218.

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