Cloning and recombinant expression of a crustin-like gene from Chinese shrimp, Fenneropenaeus chinensis

doi:10.1016/j.jbiotec.2006.08.013

IOCAS-IR > 实验海洋生物学重点实验室

	Cloning and recombinant expression of a crustin-like gene from Chinese shrimp, Fenneropenaeus chinensis
	Zhang, Jiquan ; Li, Fuhua ; Wang, Zaizhao ; Xiang, Jianhai
	2007-01-20
发表期刊	JOURNAL OF BIOTECHNOLOGY
ISSN	0168-1656
卷号	127 期号:4 页码:605-614
文章类型	Article
摘要	Antimicrobial peptides or proteins (AMPs) are proved to be one of the most important humoral factors to resist pathogen infection. As an antimicrobial protein, crustin had been described in invertebrates as a component of the innate immune system. A crustin-like gene (CruFc) was cloned from haemocytes of Chinese shrimp Fenneropenaeus chinensis by 3' and 5'-RACE PCR. The full-length cDNA consists of 523 with 405 bp open reading frame encoding 134 amino acids and the deduced peptide contains a putative signal peptide of 17 amino acids. The sequence also contains a whey-acidic protein (WAP) domain at the C-terminal. Transcripts of CruFc were mainly detected in haemocytes and gill by RT-PCR analysis. In addition, another full-length cDNA named CshFc was also cloned from haemocytes of Chinese shrimp and its inferred amino acid sequence lacks the WAP-type 'four-disulfide core' domain. The fusion proteins containing CruFc and CshFc were, respectively, produced and the antimicrobial assays revealed that the recombinant CruFc could inhibit the growth of grain-positive bacteria in vitro but the recombinant CshFc could not inhibit at the same conditions. The difference of antimicrobial activity between recombinant CruFc and CshFc provides the evidence that the four-disulfide core domain of crustin may play an important role in its biological function. (c) 2006 Elsevier B.V. All rights reserved.; Antimicrobial peptides or proteins (AMPs) are proved to be one of the most important humoral factors to resist pathogen infection. As an antimicrobial protein, crustin had been described in invertebrates as a component of the innate immune system. A crustin-like gene (CruFc) was cloned from haemocytes of Chinese shrimp Fenneropenaeus chinensis by 3' and 5'-RACE PCR. The full-length cDNA consists of 523 with 405 bp open reading frame encoding 134 amino acids and the deduced peptide contains a putative signal peptide of 17 amino acids. The sequence also contains a whey-acidic protein (WAP) domain at the C-terminal. Transcripts of CruFc were mainly detected in haemocytes and gill by RT-PCR analysis. In addition, another full-length cDNA named CshFc was also cloned from haemocytes of Chinese shrimp and its inferred amino acid sequence lacks the WAP-type 'four-disulfide core' domain. The fusion proteins containing CruFc and CshFc were, respectively, produced and the antimicrobial assays revealed that the recombinant CruFc could inhibit the growth of grain-positive bacteria in vitro but the recombinant CshFc could not inhibit at the same conditions. The difference of antimicrobial activity between recombinant CruFc and CshFc provides the evidence that the four-disulfide core domain of crustin may play an important role in its biological function. (c) 2006 Elsevier B.V. All rights reserved.
关键词	Fenneropenaeus Chinensis Crustin-like Gene Prokaryotic Expression Function Identification
学科领域	Biotechnology & Applied Microbiology
DOI	10.1016/j.jbiotec.2006.08.013
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000243704600005
引用统计	被引频次：111[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5724
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Jiquan,Li, Fuhua,Wang, Zaizhao,et al. Cloning and recombinant expression of a crustin-like gene from Chinese shrimp, Fenneropenaeus chinensis[J]. JOURNAL OF BIOTECHNOLOGY,2007,127(4):605-614.
APA	Zhang, Jiquan,Li, Fuhua,Wang, Zaizhao,&Xiang, Jianhai.(2007).Cloning and recombinant expression of a crustin-like gene from Chinese shrimp, Fenneropenaeus chinensis.JOURNAL OF BIOTECHNOLOGY,127(4),605-614.
MLA	Zhang, Jiquan,et al."Cloning and recombinant expression of a crustin-like gene from Chinese shrimp, Fenneropenaeus chinensis".JOURNAL OF BIOTECHNOLOGY 127.4(2007):605-614.

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