The lysosome and lysozyme response in Chinese shrimp Fenneropenaeus chinensis to Vibrio anguillarum and laminarin stimulation

doi:10.1016/j.jembe.2008.06.035

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	The lysosome and lysozyme response in Chinese shrimp Fenneropenaeus chinensis to Vibrio anguillarum and laminarin stimulation
	Yao, Cui-Luan 1,2; Wu, Chang-Gong 1; Xiang, Jian-Hai1 ; Li, Fuhua 1; Wang, Zhi-Yong 2; Han, Xuezhe 3
	2008-08-20
发表期刊	JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY
ISSN	0022-0981
卷号	363 期号:1-2 页码:124-129
文章类型	Article
摘要	The effect of laminarin and Vibrio anguillarum on neutral red retention (NRR) time of lysosome and lysozyme activity in blood cells was investigated in Chinese shrimp, Fenneropenaeus chinensis. In addition the variation, of total haemocyte counts (THC) and differential haemocyte counts (DHC) were dtermined simultaneously The. results showed that the lysosome membrane stability was significantly elongated with the longest NRR time for 180 min at 3 h after lamianrin injection, (p<0.05). The lysozyme activity of haemocytes showed a moderate increase simultaneously. THC also increased and with a highest percentage of semi-granular cell counts at 3 h after laminarin injection. However, the NRR time sharply decreased with the shortest NRR time for 13 min at 1.5 h after V. anguillarum injection. Compared to the control group, the lysozyme activity obviously increased after injection, which was demonstrated at 1.5 h though the THC decreased. The percentage of hyaline cells increased obviously after V. anguillarum injection (p<0.05). The results suggested that the shrimp immune status could be evaluated by using lysosome membrane stability and lysozyme activity. (C) 2008 Elsevier B.V. All rights reserved.; The effect of laminarin and Vibrio anguillarum on neutral red retention (NRR) time of lysosome and lysozyme activity in blood cells was investigated in Chinese shrimp, Fenneropenaeus chinensis. In addition the variation, of total haemocyte counts (THC) and differential haemocyte counts (DHC) were dtermined simultaneously The. results showed that the lysosome membrane stability was significantly elongated with the longest NRR time for 180 min at 3 h after lamianrin injection, (p<0.05). The lysozyme activity of haemocytes showed a moderate increase simultaneously. THC also increased and with a highest percentage of semi-granular cell counts at 3 h after laminarin injection. However, the NRR time sharply decreased with the shortest NRR time for 13 min at 1.5 h after V. anguillarum injection. Compared to the control group, the lysozyme activity obviously increased after injection, which was demonstrated at 1.5 h though the THC decreased. The percentage of hyaline cells increased obviously after V. anguillarum injection (p<0.05). The results suggested that the shrimp immune status could be evaluated by using lysosome membrane stability and lysozyme activity. (C) 2008 Elsevier B.V. All rights reserved.
关键词	Fenneropenaeus Chinensis Haemocyte Laminarin Lysosome Lysozyme Neutral Red Retention Assay Vibrio Anguillarum
学科领域	Ecology ; Marine & Freshwater Biology
DOI	10.1016/j.jembe.2008.06.035
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000259419800017
引用统计	被引频次：72[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5708
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Jimei Univ, Coll Fisheries, Fujian Prov Univ, Key Lab Sci & Tech Aquaculture & Food Safety, Xiamen 361021, Peoples R China 3.Sci Press, Beijing 100080, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Yao, Cui-Luan,Wu, Chang-Gong,Xiang, Jian-Hai,et al. The lysosome and lysozyme response in Chinese shrimp Fenneropenaeus chinensis to Vibrio anguillarum and laminarin stimulation[J]. JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY,2008,363(1-2):124-129.
APA	Yao, Cui-Luan,Wu, Chang-Gong,Xiang, Jian-Hai,Li, Fuhua,Wang, Zhi-Yong,&Han, Xuezhe.(2008).The lysosome and lysozyme response in Chinese shrimp Fenneropenaeus chinensis to Vibrio anguillarum and laminarin stimulation.JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY,363(1-2),124-129.
MLA	Yao, Cui-Luan,et al."The lysosome and lysozyme response in Chinese shrimp Fenneropenaeus chinensis to Vibrio anguillarum and laminarin stimulation".JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY 363.1-2(2008):124-129.

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