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| Spectral changes of C-phycocyanin with different molar ratios of SPDP | |
| Yan Shi-gan1,3; Chen Xiu-lan1; Zhang Xi-ying1; Zhou Bai-cheng1,2; Zhang Yu-zhong1 | |
| 2008-05-01 | |
| 发表期刊 | SPECTROSCOPY AND SPECTRAL ANALYSIS
 |
| ISSN | 1000-0593 |
| 卷号 | 28期号:5页码:1115-1117 |
| 文章类型 | Article |
| 摘要 | Pure C-phycocyanin was prepared from Spirulina platensis using one-step anion-exchange chromatography. The C-PC obtained was with an absorption maximum at 620 nm and a fluorescence emission maximum at 640 nm when excited by 580 nm. SPDP is an excellent heterobifunctional crosslinker for thiolating amines. Different molar ratios of SPDP have remarkable influence on the absorption and fluorescence spectra of C-phycocyanin. The absorption maximum and fluorescence emission maximum both decreased and blue-shifted from 640 run to 630 nm as the molar ratios of SPDP increased. It was found that the molar ratios of SPDP to C-phycocyanin was not more than 100 was appropriate to being conjugated with other biomolecules from the absorption and fluorescence spectra of C-phycocyanin.; Pure C-phycocyanin was prepared from Spirulina platensis using one-step anion-exchange chromatography. The C-PC obtained was with an absorption maximum at 620 nm and a fluorescence emission maximum at 640 nm when excited by 580 nm. SPDP is an excellent heterobifunctional crosslinker for thiolating amines. Different molar ratios of SPDP have remarkable influence on the absorption and fluorescence spectra of C-phycocyanin. The absorption maximum and fluorescence emission maximum both decreased and blue-shifted from 640 run to 630 nm as the molar ratios of SPDP increased. It was found that the molar ratios of SPDP to C-phycocyanin was not more than 100 was appropriate to being conjugated with other biomolecules from the absorption and fluorescence spectra of C-phycocyanin. |
| 关键词 | C-phycocyanin Spdp Spectrum |
| 学科领域 | Spectroscopy |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000257972000036 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/5706 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 3.Shandong Key Lab Anim Dis Control & Breeding, Jinan 250100, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yan Shi-gan,Chen Xiu-lan,Zhang Xi-ying,et al. Spectral changes of C-phycocyanin with different molar ratios of SPDP[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2008,28(5):1115-1117. |
| APA | Yan Shi-gan,Chen Xiu-lan,Zhang Xi-ying,Zhou Bai-cheng,&Zhang Yu-zhong.(2008).Spectral changes of C-phycocyanin with different molar ratios of SPDP.SPECTROSCOPY AND SPECTRAL ANALYSIS,28(5),1115-1117. |
| MLA | Yan Shi-gan,et al."Spectral changes of C-phycocyanin with different molar ratios of SPDP".SPECTROSCOPY AND SPECTRAL ANALYSIS 28.5(2008):1115-1117. |
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