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Combinational biosynthesis of a fluorescent cyanobacterial holo-alpha-allophycocyanin in Escherichia coli
Yang, Yu1,2; Ge, Baosheng3; Guan, Xiangyu4; Zhang, Weijie1,2; Qin, Song1
2008-06-01
发表期刊BIOTECHNOLOGY LETTERS
ISSN0141-5492
卷号30期号:6页码:1001-1004
文章类型Article
摘要Allophycocyanin ( APC) is a phycobiliprotein with various biological and pharmacological properties. An expression vector containing five essential genes in charge of biosynthesis of cyanobacterial APC holo-alpha subunit ( holo- ApcA) was constructed, resulting in over- expression of a fluorescent holo- ApcA in E. coli. After being cultured for 16 h, the dry cell density reached 22.5 gl(-1), and the expression of holo- HT- ApcA was up to 1 gl(-1) broth. The recombinant protein showed similar spectral features to native APC.; Allophycocyanin ( APC) is a phycobiliprotein with various biological and pharmacological properties. An expression vector containing five essential genes in charge of biosynthesis of cyanobacterial APC holo-alpha subunit ( holo- ApcA) was constructed, resulting in over- expression of a fluorescent holo- ApcA in E. coli. After being cultured for 16 h, the dry cell density reached 22.5 gl(-1), and the expression of holo- HT- ApcA was up to 1 gl(-1) broth. The recombinant protein showed similar spectral features to native APC.
关键词Allophycocyanin Escherichia Coli Fluorescence Recombinant Protein
学科领域Biotechnology & Applied Microbiology
DOI10.1007/s10529-008-9644-2
URL查看原文
收录类别SCI
语种英语
WOS记录号WOS:000255190300005
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被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/5702
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
3.China Univ Petr, Ctr Biotechnol & Bioengn, Qingdao 266555, Peoples R China
4.Ocean Univ China, Qingdao 266003, Peoples R China
第一作者单位中国科学院海洋研究所
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GB/T 7714
Yang, Yu,Ge, Baosheng,Guan, Xiangyu,et al. Combinational biosynthesis of a fluorescent cyanobacterial holo-alpha-allophycocyanin in Escherichia coli[J]. BIOTECHNOLOGY LETTERS,2008,30(6):1001-1004.
APA Yang, Yu,Ge, Baosheng,Guan, Xiangyu,Zhang, Weijie,&Qin, Song.(2008).Combinational biosynthesis of a fluorescent cyanobacterial holo-alpha-allophycocyanin in Escherichia coli.BIOTECHNOLOGY LETTERS,30(6),1001-1004.
MLA Yang, Yu,et al."Combinational biosynthesis of a fluorescent cyanobacterial holo-alpha-allophycocyanin in Escherichia coli".BIOTECHNOLOGY LETTERS 30.6(2008):1001-1004.
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