Molecular analysis of the fur (ferric uptake regulator) gene of a pathogenic Edwardsiella tarda strain

doi:10.1007/s12275-008-0038-x

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	Molecular analysis of the fur (ferric uptake regulator) gene of a pathogenic Edwardsiella tarda strain
	Wang, Fang 1,2; Cheng, Shuang 1,2; Sun, Kun 1,2; Sun, Li 1
	2008-06-01
发表期刊	JOURNAL OF MICROBIOLOGY
ISSN	1225-8873
卷号	46 期号:3 页码:350-355
文章类型	Article
摘要	The gene encoding the Edwardsiella tarda ferric uptake regulator (Fur(Et)) was cloned from a pathogenic E. tarda strain isolated from diseased fish. Fur(Et) shares 90% overall sequence identity with the Escherichia coli Fur (Fur(Ec)) and was able to complement the mutant phenotype of a fur(Ec)-defective E. coli strain. Mutational analysis indicated that C92S and C95S mutations inactivated Fur(Et) whereas E112K mutation resulted in a superactive Fur(Et) variant. Fur(Et) negatively regulated its own expression; interruption of this regulation impaired bacterial growth, altered the production of certain outer membrane proteins, and attenuated bacterial virulence.; The gene encoding the Edwardsiella tarda ferric uptake regulator (Fur(Et)) was cloned from a pathogenic E. tarda strain isolated from diseased fish. Fur(Et) shares 90% overall sequence identity with the Escherichia coli Fur (Fur(Ec)) and was able to complement the mutant phenotype of a fur(Ec)-defective E. coli strain. Mutational analysis indicated that C92S and C95S mutations inactivated Fur(Et) whereas E112K mutation resulted in a superactive Fur(Et) variant. Fur(Et) negatively regulated its own expression; interruption of this regulation impaired bacterial growth, altered the production of certain outer membrane proteins, and attenuated bacterial virulence.
关键词	Edwardsiella Tarda Fur Autoregulation Virulence Mutation
学科领域	Microbiology
DOI	10.1007/s12275-008-0038-x
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000257240800017
引用统计	被引频次：33[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5672
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wang, Fang,Cheng, Shuang,Sun, Kun,et al. Molecular analysis of the fur (ferric uptake regulator) gene of a pathogenic Edwardsiella tarda strain[J]. JOURNAL OF MICROBIOLOGY,2008,46(3):350-355.
APA	Wang, Fang,Cheng, Shuang,Sun, Kun,&Sun, Li.(2008).Molecular analysis of the fur (ferric uptake regulator) gene of a pathogenic Edwardsiella tarda strain.JOURNAL OF MICROBIOLOGY,46(3),350-355.
MLA	Wang, Fang,et al."Molecular analysis of the fur (ferric uptake regulator) gene of a pathogenic Edwardsiella tarda strain".JOURNAL OF MICROBIOLOGY 46.3(2008):350-355.

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