Inter-simple sequence repeat (ISSR) analysis of genetic variation of Chondrus crispus populations from North Atlantic

doi:10.1016/j.aquabot.2007.10.001

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	Inter-simple sequence repeat (ISSR) analysis of genetic variation of Chondrus crispus populations from North Atlantic
	Wang, Xiuliang 1; Zhao, Fengjuan 1; Hu, Zimin 2; Critchley, Alan T.3; Morrell, Steve L.; Duan, Delin 1
	2008-02-01
发表期刊	AQUATIC BOTANY
ISSN	0304-3770
卷号	88 期号:2 页码:154-159
文章类型	Article
摘要	ISSR analysis was used to investigate genetic variations of 184 haploid and diploid samples from nine North Atlantic Chondrus crispus Stackhouse populations and one outgroup Yellow Sea Chondrus ocellatus Holmes population. Twenty-two of 50 primers were selected and 163 loci were scored for genetic diversity analysis. Genetic diversity varied among populations, percentage of polymorphic bands (PPB) ranged from 27.0 to 55.8%, H(Nei's genetic diversity) ranged from 0.11 to 0.20 and I(Shannon's information index) ranged from 0.16 to 0.30. Estimators PPB, H and I had similar values in intra-population genetic diversity, regardless of calculation methods. Analysis of molecular variance (AMOVA) apportioned inter-population and intra-population variations for C crispus, showing more genetic variance (56.5%) occurred in intra-population, and 43.5% variation among nine populations. The Mantel test suggested that genetic differentiation between nine C. crispus populations was closely related with geographic distances (R = 0.78, P = 0.002). Results suggest that, on larger distance scale (ca. > 1000 km), ISSR analysis is useful for determining genetic differentiations of C crispus populations including morphologically inseparable haploid and diploid individuals. (c) 2007 Elsevier B.V. All rights reserved.; ISSR analysis was used to investigate genetic variations of 184 haploid and diploid samples from nine North Atlantic Chondrus crispus Stackhouse populations and one outgroup Yellow Sea Chondrus ocellatus Holmes population. Twenty-two of 50 primers were selected and 163 loci were scored for genetic diversity analysis. Genetic diversity varied among populations, percentage of polymorphic bands (PPB) ranged from 27.0 to 55.8%, H(Nei's genetic diversity) ranged from 0.11 to 0.20 and I(Shannon's information index) ranged from 0.16 to 0.30. Estimators PPB, H and I had similar values in intra-population genetic diversity, regardless of calculation methods. Analysis of molecular variance (AMOVA) apportioned inter-population and intra-population variations for C crispus, showing more genetic variance (56.5%) occurred in intra-population, and 43.5% variation among nine populations. The Mantel test suggested that genetic differentiation between nine C. crispus populations was closely related with geographic distances (R = 0.78, P = 0.002). Results suggest that, on larger distance scale (ca. > 1000 km), ISSR analysis is useful for determining genetic differentiations of C crispus populations including morphologically inseparable haploid and diploid individuals. (c) 2007 Elsevier B.V. All rights reserved.
关键词	Rhodophyta Chondrus Crispus Stackhouse Population Genetic Variation Geographic Distance Issr Amova Mantel Test
学科领域	Plant Sciences ; Marine & Freshwater Biology
DOI	10.1016/j.aquabot.2007.10.001
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000253021700010
引用统计	被引频次：37[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5666
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Ocean Univ China, Div Life Sci & Technol, Qingdao 266003, Peoples R China 3.Acadian Seaplants Ltd, Dartmouth, NS B3B 1X8, Canada
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wang, Xiuliang,Zhao, Fengjuan,Hu, Zimin,et al. Inter-simple sequence repeat (ISSR) analysis of genetic variation of Chondrus crispus populations from North Atlantic[J]. AQUATIC BOTANY,2008,88(2):154-159.
APA	Wang, Xiuliang,Zhao, Fengjuan,Hu, Zimin,Critchley, Alan T.,Morrell, Steve L.,&Duan, Delin.(2008).Inter-simple sequence repeat (ISSR) analysis of genetic variation of Chondrus crispus populations from North Atlantic.AQUATIC BOTANY,88(2),154-159.
MLA	Wang, Xiuliang,et al."Inter-simple sequence repeat (ISSR) analysis of genetic variation of Chondrus crispus populations from North Atlantic".AQUATIC BOTANY 88.2(2008):154-159.

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