Large-scale recovery of C-phycocyanin from Spirulina platensis using expanded bed adsorption chromatography

doi:10.1016/j.jchromb.2006.11.043

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	Large-scale recovery of C-phycocyanin from Spirulina platensis using expanded bed adsorption chromatography
	Niu, Han-Feng ; Wang, Guang-Ce ; Lin, Xiang-zhi ; Zhou, Bai-Cheng
	2007-05-01
发表期刊	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN	1570-0232
卷号	850 期号:1-2 页码:267-276
文章类型	Article
摘要	C-phycocyanin was purified on a large scale by a combination of expanded bed adsorption, anion-exchange chromatography and hydroxyapatite chromatography from inferior Spirulina platensis that cannot be used for human consumption. First, phycobiliproteins were extracted by a simple, scaleable method and then were recovered by Phenyl-Sepharose chromatography in an expanded bed column. The purity (the A(620)/A(280) ratio) of C-phycocyanin isolated with STREAMLINE (TM) Column was up to 2.87, and the yield was as high as 31 mg/g of dried S. platensis. After the first step, we used conventional anion-exchange chromatography for the purification steps, with a yield of 7.7 mg/g of dried S. platensis at a purity greater than 3.2 and with an A(620)/A(650) index higher than 5.0. The fractions from anion-exchange chromatography with a level of purity that did not conform to the above standard were subjected to hydroxyapatite chromatography, with a C-PC yield of 4.45 mg/g of dried S. platensis with a purity greater than 3.2. The protein from both purification methods showed one absolute absorption peak at 620 nm and a fluorescence maximum at 650 nm, which is consistent with the typical spectrum of C-phycocyanin. SDS-PAGE gave two bands corresponding to 21 and 18 kDa. In-gel digestion and LC-ESI-MS showed that the protein is C-phycocyanin. (c) 2006 Elsevier B.V. All rights reserved.; C-phycocyanin was purified on a large scale by a combination of expanded bed adsorption, anion-exchange chromatography and hydroxyapatite chromatography from inferior Spirulina platensis that cannot be used for human consumption. First, phycobiliproteins were extracted by a simple, scaleable method and then were recovered by Phenyl-Sepharose chromatography in an expanded bed column. The purity (the A(620)/A(280) ratio) of C-phycocyanin isolated with STREAMLINE (TM) Column was up to 2.87, and the yield was as high as 31 mg/g of dried S. platensis. After the first step, we used conventional anion-exchange chromatography for the purification steps, with a yield of 7.7 mg/g of dried S. platensis at a purity greater than 3.2 and with an A(620)/A(650) index higher than 5.0. The fractions from anion-exchange chromatography with a level of purity that did not conform to the above standard were subjected to hydroxyapatite chromatography, with a C-PC yield of 4.45 mg/g of dried S. platensis with a purity greater than 3.2. The protein from both purification methods showed one absolute absorption peak at 620 nm and a fluorescence maximum at 650 nm, which is consistent with the typical spectrum of C-phycocyanin. SDS-PAGE gave two bands corresponding to 21 and 18 kDa. In-gel digestion and LC-ESI-MS showed that the protein is C-phycocyanin. (c) 2006 Elsevier B.V. All rights reserved.
关键词	Spirulina Platensis Purification C-phycocyanin Streamline (Tm) Column Anion-exchange Chromatography Hydroxyapatite Chromatography
学科领域	Biochemical Research Methods ; Chemistry, Analytical
DOI	10.1016/j.jchromb.2006.11.043
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000246703500034
引用统计	被引频次：73[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5620
专题	实验海洋生物学重点实验室
作者单位	1.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin 300457, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 4.State Ocean Adm Peoples Republ China, Inst Oceanog 3, Xiamen 361005, Peoples R China
推荐引用方式 GB/T 7714	Niu, Han-Feng,Wang, Guang-Ce,Lin, Xiang-zhi,et al. Large-scale recovery of C-phycocyanin from Spirulina platensis using expanded bed adsorption chromatography[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2007,850(1-2):267-276.
APA	Niu, Han-Feng,Wang, Guang-Ce,Lin, Xiang-zhi,&Zhou, Bai-Cheng.(2007).Large-scale recovery of C-phycocyanin from Spirulina platensis using expanded bed adsorption chromatography.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,850(1-2),267-276.
MLA	Niu, Han-Feng,et al."Large-scale recovery of C-phycocyanin from Spirulina platensis using expanded bed adsorption chromatography".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 850.1-2(2007):267-276.

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