Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Effect of iron on growth and lipid accumulation in Chlorella vulgaris | |
| Liu, Zhi-Yuan1,3; Wang, Guang-Ce1,2; Zhou, Bai-Cheng1 | |
| 2008-07-01 | |
| Source Publication | BIORESOURCE TECHNOLOGY
 |
| ISSN | 0960-8524 |
| Volume | 99Issue:11Pages:4717-4722 |
| Subtype | Article |
| Abstract | The economic feasibility of algal mass culture for biodiesel production is enhanced by the increase in biomass productivity and storage lipids. Effect of iron on growth and lipid accumulation in marine microalgae Chlorella vulgaris were investigated. In experiment I, supplementing the growth media with chelated FeCl(3) in the late growth phase increased the final cell density but did not induce lipid accumulation in cells. In experiment II, cells in the late-exponential growth phase were collected by centrifugation and re-inoculated into new media supplemented with five levels of Fe(3+) concentration. Total lipid content in cultures supplemented with 1.2 x 10(-5) mol L(-1) FeCl(3) was up to 56.6% biomass by dry weight and was 3-7-fold that in other media supplemented with lower iron concentration. Moreover, a simple and rapid method determining the lipid accumulation in C. vulgaris with spectrofluorimetry was developed. (c) 2007 Elsevier Ltd. All rights reserved.; The economic feasibility of algal mass culture for biodiesel production is enhanced by the increase in biomass productivity and storage lipids. Effect of iron on growth and lipid accumulation in marine microalgae Chlorella vulgaris were investigated. In experiment I, supplementing the growth media with chelated FeCl3 in the late growth phase increased the final cell density but did not induce lipid accumulation in cells. In experiment II, cells in the late-exponential growth phase were collected by centrifugation and re-inoculated into new media supplemented with five levels of Fe3+ concentration. Total lipid content in cultures supplemented with 1.2 x 10(-5) mol L-1 FeCl3 was up to 56.6% biomass by dry weight and was 3-7-fold that in other media supplemented with lower iron concentration. Moreover, a simple and rapid method determining the lipid accumulation in C. vulgaris with spectrofluorimetry was developed. (c) 2007 Elsevier Ltd. All rights reserved. |
| Keyword | Lipid Fecl(3) Microalgae Nile Red Spectrofluorimetry |
| Subject Area | Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| DOI | 10.1016/j.biortech.2007.09.073 |
| URL | 查看原文 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000256050500026 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/5602 |
| Collection | 实验海洋生物学重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Tianjin Univ Sci Technol, Tianjin 300222, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| First Author Affilication | Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Liu, Zhi-Yuan,Wang, Guang-Ce,Zhou, Bai-Cheng. Effect of iron on growth and lipid accumulation in Chlorella vulgaris[J]. BIORESOURCE TECHNOLOGY,2008,99(11):4717-4722. |
| APA | Liu, Zhi-Yuan,Wang, Guang-Ce,&Zhou, Bai-Cheng.(2008).Effect of iron on growth and lipid accumulation in Chlorella vulgaris.BIORESOURCE TECHNOLOGY,99(11),4717-4722. |
| MLA | Liu, Zhi-Yuan,et al."Effect of iron on growth and lipid accumulation in Chlorella vulgaris".BIORESOURCE TECHNOLOGY 99.11(2008):4717-4722. |
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