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Effect of iron on growth and lipid accumulation in Chlorella vulgaris
Liu, Zhi-Yuan1,3; Wang, Guang-Ce1,2; Zhou, Bai-Cheng1
2008-07-01
Source PublicationBIORESOURCE TECHNOLOGY
ISSN0960-8524
Volume99Issue:11Pages:4717-4722
SubtypeArticle
AbstractThe economic feasibility of algal mass culture for biodiesel production is enhanced by the increase in biomass productivity and storage lipids. Effect of iron on growth and lipid accumulation in marine microalgae Chlorella vulgaris were investigated. In experiment I, supplementing the growth media with chelated FeCl(3) in the late growth phase increased the final cell density but did not induce lipid accumulation in cells. In experiment II, cells in the late-exponential growth phase were collected by centrifugation and re-inoculated into new media supplemented with five levels of Fe(3+) concentration. Total lipid content in cultures supplemented with 1.2 x 10(-5) mol L(-1) FeCl(3) was up to 56.6% biomass by dry weight and was 3-7-fold that in other media supplemented with lower iron concentration. Moreover, a simple and rapid method determining the lipid accumulation in C. vulgaris with spectrofluorimetry was developed. (c) 2007 Elsevier Ltd. All rights reserved.; The economic feasibility of algal mass culture for biodiesel production is enhanced by the increase in biomass productivity and storage lipids. Effect of iron on growth and lipid accumulation in marine microalgae Chlorella vulgaris were investigated. In experiment I, supplementing the growth media with chelated FeCl3 in the late growth phase increased the final cell density but did not induce lipid accumulation in cells. In experiment II, cells in the late-exponential growth phase were collected by centrifugation and re-inoculated into new media supplemented with five levels of Fe3+ concentration. Total lipid content in cultures supplemented with 1.2 x 10(-5) mol L-1 FeCl3 was up to 56.6% biomass by dry weight and was 3-7-fold that in other media supplemented with lower iron concentration. Moreover, a simple and rapid method determining the lipid accumulation in C. vulgaris with spectrofluorimetry was developed. (c) 2007 Elsevier Ltd. All rights reserved.
KeywordLipid Fecl(3) Microalgae Nile Red Spectrofluorimetry
Subject AreaAgricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
DOI10.1016/j.biortech.2007.09.073
URL查看原文
Indexed BySCI
Language英语
WOS IDWOS:000256050500026
Citation statistics
Cited Times:421[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/5602
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Tianjin Univ Sci Technol, Tianjin 300222, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Liu, Zhi-Yuan,Wang, Guang-Ce,Zhou, Bai-Cheng. Effect of iron on growth and lipid accumulation in Chlorella vulgaris[J]. BIORESOURCE TECHNOLOGY,2008,99(11):4717-4722.
APA Liu, Zhi-Yuan,Wang, Guang-Ce,&Zhou, Bai-Cheng.(2008).Effect of iron on growth and lipid accumulation in Chlorella vulgaris.BIORESOURCE TECHNOLOGY,99(11),4717-4722.
MLA Liu, Zhi-Yuan,et al."Effect of iron on growth and lipid accumulation in Chlorella vulgaris".BIORESOURCE TECHNOLOGY 99.11(2008):4717-4722.
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