Sequence analysis of Arthrospira maxima based on fosmid library

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	Sequence analysis of Arthrospira maxima based on fosmid library
	Ling, Na; Mao, Yunxiang; Zhang, Xuecheng; Mo, Zhaolan; Wang, Guangce; Liu, Wei
	2007
发表期刊	JOURNAL OF APPLIED PHYCOLOGY
ISSN	0921-8971
卷号	19 期号:4 页码:333-346
摘要	Arthrospira (Spirulina) (Setchell& Gardner) is an important cyanobacterium not only in its nutritional potential but in its special biological characteristics. An unbiased fosmid library of Arthrospira maxima FACHB438 that contains 4300 clones was constructed. The size distribution of insert fragments is from 15.5 to 48.9 kb and the average size is 37.6 kb. The recombination frequency is 100%. Therefore the library is 29.9 equivalents to the Arthrospira genome size of 5.4 Mb. A total of 719 sample clones were randomly chosen from the library and 602 available sequences, which consisted of 307,547 bases, covering 5.70% of the whole genome. The codon usage of A. maxima was not strongly biased. GC content at the first position of codons (46.9%) was higher than the second (39.8%) and the third (45.5%) positions. GC content of the genome was 43.6%. Of these sequences, 287 (47.7%) showed high similarities to known genes, 63 (10.5%) to hypothetical genes and the remaining 252 (41.8%) had no significant similarities. The assigned genes were classified into 22 categories with respect to different biological roles. Remarkably, the high presence of 25 sequences (4.2%) encoding reverse transcriptase indicates the RT gene may have multiple copies in the A. maxima genome and might play an important role in the evolutionary history and metabolic regulation. In addition, the sequences encoding the ATP-binding cassette transport system and the two-component signal transduction system were the second and third most frequent genes, respectively. These genomic features provide some clues as to the mechanisms by which this organism adapts to the high concentration of bicarbonate and to the high pH environment.
关键词	Cyanobacterium Spirulina-platensis Rna-linked Msdna Reverse-transcriptase Myxococcus-xanthus Branched Rna Escherichia-coli Abc Transporters Genome Strain Identification
学科领域	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
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收录类别	sci
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5596
专题	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Ling, Na,Mao, Yunxiang,Zhang, Xuecheng,et al. Sequence analysis of Arthrospira maxima based on fosmid library[J]. JOURNAL OF APPLIED PHYCOLOGY,2007,19(4):333-346.
APA	Ling, Na,Mao, Yunxiang,Zhang, Xuecheng,Mo, Zhaolan,Wang, Guangce,&Liu, Wei.(2007).Sequence analysis of Arthrospira maxima based on fosmid library.JOURNAL OF APPLIED PHYCOLOGY,19(4),333-346.
MLA	Ling, Na,et al."Sequence analysis of Arthrospira maxima based on fosmid library".JOURNAL OF APPLIED PHYCOLOGY 19.4(2007):333-346.

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