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Purification of High-quality Plastid DNA from the Brown Alga Laminaria japonica Sporophytes
Fu, Wandong1,2; Yao, Jianting1,2; Wang, Xiuliang1; Liu, Fuli1,2; Chong, Zhuo1,2; Duan, Delin1
2008-06-01
发表期刊PLANT MOLECULAR BIOLOGY REPORTER
ISSN0735-9640
卷号26期号:2页码:114-120
文章类型Article
摘要Extracting DNA from a variety of algae is rather difficult because of high levels of polysaccharides, tannins, and phenolics as these interfere with DNA isolation and downstream applications. High-quality plastid DNA (ptDNA) purification is particularly difficult because of its small proportion in total genomic DNA. This report describes an improved protocol for ptDNA purification that efficiently produces high-quality ptDNA from sporophytes of Laminaria japonica and several other algae. This improved protocol simplifies procedures for ptDNA purification and improves yield to 150-200 mu g of ptDNA per 100 g of frozen algal tissue. Polymerase chain reaction (PCR) amplification of conserved sequences has been used to verify purity of the ptDNA product.; Extracting DNA from a variety of algae is rather difficult because of high levels of polysaccharides, tannins, and phenolics as these interfere with DNA isolation and downstream applications. High-quality plastid DNA (ptDNA) purification is particularly difficult because of its small proportion in total genomic DNA. This report describes an improved protocol for ptDNA purification that efficiently produces high-quality ptDNA from sporophytes of Laminaria japonica and several other algae. This improved protocol simplifies procedures for ptDNA purification and improves yield to 150-200 mu g of ptDNA per 100 g of frozen algal tissue. Polymerase chain reaction (PCR) amplification of conserved sequences has been used to verify purity of the ptDNA product.
关键词Brown Alga Laminaria Japonica Plastid Dna Ultracentrifugation
学科领域Biochemical Research Methods ; Plant Sciences
DOI10.1007/s11105-008-0028-5
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收录类别SCI
语种英语
WOS记录号WOS:000259962500004
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被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/5548
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
第一作者单位中国科学院海洋研究所
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Fu, Wandong,Yao, Jianting,Wang, Xiuliang,et al. Purification of High-quality Plastid DNA from the Brown Alga Laminaria japonica Sporophytes[J]. PLANT MOLECULAR BIOLOGY REPORTER,2008,26(2):114-120.
APA Fu, Wandong,Yao, Jianting,Wang, Xiuliang,Liu, Fuli,Chong, Zhuo,&Duan, Delin.(2008).Purification of High-quality Plastid DNA from the Brown Alga Laminaria japonica Sporophytes.PLANT MOLECULAR BIOLOGY REPORTER,26(2),114-120.
MLA Fu, Wandong,et al."Purification of High-quality Plastid DNA from the Brown Alga Laminaria japonica Sporophytes".PLANT MOLECULAR BIOLOGY REPORTER 26.2(2008):114-120.
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