Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Purification of High-quality Plastid DNA from the Brown Alga Laminaria japonica Sporophytes | |
Fu, Wandong1,2; Yao, Jianting1,2; Wang, Xiuliang1; Liu, Fuli1,2; Chong, Zhuo1,2; Duan, Delin1 | |
2008-06-01 | |
发表期刊 | PLANT MOLECULAR BIOLOGY REPORTER |
ISSN | 0735-9640 |
卷号 | 26期号:2页码:114-120 |
文章类型 | Article |
摘要 | Extracting DNA from a variety of algae is rather difficult because of high levels of polysaccharides, tannins, and phenolics as these interfere with DNA isolation and downstream applications. High-quality plastid DNA (ptDNA) purification is particularly difficult because of its small proportion in total genomic DNA. This report describes an improved protocol for ptDNA purification that efficiently produces high-quality ptDNA from sporophytes of Laminaria japonica and several other algae. This improved protocol simplifies procedures for ptDNA purification and improves yield to 150-200 mu g of ptDNA per 100 g of frozen algal tissue. Polymerase chain reaction (PCR) amplification of conserved sequences has been used to verify purity of the ptDNA product.; Extracting DNA from a variety of algae is rather difficult because of high levels of polysaccharides, tannins, and phenolics as these interfere with DNA isolation and downstream applications. High-quality plastid DNA (ptDNA) purification is particularly difficult because of its small proportion in total genomic DNA. This report describes an improved protocol for ptDNA purification that efficiently produces high-quality ptDNA from sporophytes of Laminaria japonica and several other algae. This improved protocol simplifies procedures for ptDNA purification and improves yield to 150-200 mu g of ptDNA per 100 g of frozen algal tissue. Polymerase chain reaction (PCR) amplification of conserved sequences has been used to verify purity of the ptDNA product. |
关键词 | Brown Alga Laminaria Japonica Plastid Dna Ultracentrifugation |
学科领域 | Biochemical Research Methods ; Plant Sciences |
DOI | 10.1007/s11105-008-0028-5 |
URL | 查看原文 |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000259962500004 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/5548 |
专题 | 实验海洋生物学重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
第一作者单位 | 中国科学院海洋研究所 |
推荐引用方式 GB/T 7714 | Fu, Wandong,Yao, Jianting,Wang, Xiuliang,et al. Purification of High-quality Plastid DNA from the Brown Alga Laminaria japonica Sporophytes[J]. PLANT MOLECULAR BIOLOGY REPORTER,2008,26(2):114-120. |
APA | Fu, Wandong,Yao, Jianting,Wang, Xiuliang,Liu, Fuli,Chong, Zhuo,&Duan, Delin.(2008).Purification of High-quality Plastid DNA from the Brown Alga Laminaria japonica Sporophytes.PLANT MOLECULAR BIOLOGY REPORTER,26(2),114-120. |
MLA | Fu, Wandong,et al."Purification of High-quality Plastid DNA from the Brown Alga Laminaria japonica Sporophytes".PLANT MOLECULAR BIOLOGY REPORTER 26.2(2008):114-120. |
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