Identification of a novel DNA methyltransferase 2 from the brine shrimp, Artemia franciscana

doi:10.1016/j.cbpb.2007.01.024

IOCAS-IR > 实验海洋生物学重点实验室

	Identification of a novel DNA methyltransferase 2 from the brine shrimp, Artemia franciscana
	Feng, Chen-Zhuo ; Zhu, Xiao-Jing ; Dai, Zhong-Min ; Liu, Feng-Qi ; Xiang, Jian-Hai; Yang, Wei-Jun
	2007-06-01
发表期刊	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
ISSN	1096-4959
卷号	147 期号:2 页码:191-198
文章类型	Article
摘要	DNA methyltransferase 2 (Dnmt2) is a dual-specificity DNA methyltransferase, which contains a weak DNA methyltransferase and novel tRNA methyltransferase activity. However, its biological function is still enigmatic. To elucidate the expression profiles of Dnmt2 in Artemia franciscana, we isolated the gene encoding a Dnmt2 from A. franciscana and named it as AfDnmt2. The cDNA of AfDnmt2 contained a 1140-bp open reading frame that encoded a putative Dnmt2 protein of 379 amino acids exhibiting 32%similar to 39% identities with other known Dnmt2 homologs. This is the first report of a DNA methyltransferase gene in Crustacean. By using semi-quantitative RT-PCR, A)Dnmt2 was found to be expressed through all developmental stages and its expression increased during resumption of diapause cysts development. Southern blot analysis indicated the presence of multiple copies of AfDnmt2 genes in A. franciscana. (C) 2007 Published by Elsevier Inc.; DNA methyltransferase 2 (Dnmt2) is a dual-specificity DNA methyltransferase, which contains a weak DNA methyltransferase and novel tRNA methyltransferase activity. However, its biological function is still enigmatic. To elucidate the expression profiles of Dnmt2 in Artemia franciscana, we isolated the gene encoding a Dnmt2 from A. franciscana and named it as AfDnmt2. The cDNA of AfDnmt2 contained a 1140-bp open reading frame that encoded a putative Dnmt2 protein of 379 amino acids exhibiting 32%similar to 39% identities with other known Dnmt2 homologs. This is the first report of a DNA methyltransferase gene in Crustacean. By using semi-quantitative RT-PCR, A)Dnmt2 was found to be expressed through all developmental stages and its expression increased during resumption of diapause cysts development. Southern blot analysis indicated the presence of multiple copies of AfDnmt2 genes in A. franciscana. (C) 2007 Published by Elsevier Inc.
关键词	Dnmt2 Artemia Franciscana Diapause Cysts Development
学科领域	Biochemistry & Molecular Biology ; Zoology
DOI	10.1016/j.cbpb.2007.01.024
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000246544100006
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5542
专题	实验海洋生物学重点实验室
作者单位	1.Zhejiang Univ, Inst Cell Biol & Genet, Coll Life Sci, Hangzhou 310058, Zhejiang, Peoples R China 2.Nankai Univ, Coll Life Sci, Tianjin 300071, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Feng, Chen-Zhuo,Zhu, Xiao-Jing,Dai, Zhong-Min,et al. Identification of a novel DNA methyltransferase 2 from the brine shrimp, Artemia franciscana[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2007,147(2):191-198.
APA	Feng, Chen-Zhuo,Zhu, Xiao-Jing,Dai, Zhong-Min,Liu, Feng-Qi,Xiang, Jian-Hai,&Yang, Wei-Jun.(2007).Identification of a novel DNA methyltransferase 2 from the brine shrimp, Artemia franciscana.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,147(2),191-198.
MLA	Feng, Chen-Zhuo,et al."Identification of a novel DNA methyltransferase 2 from the brine shrimp, Artemia franciscana".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 147.2(2007):191-198.

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