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The expression of AmphiTCTP, a TCTP orthologous gene in amphioxus related to the development of notochord and somites
Chen, Zhongke; Zhang, Hongwei; Yang, Hui; Huang, Xiangwei; Zhang, Xiaohui; Zhang, Peijun
2007-07-01
发表期刊COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
ISSN1096-4959
卷号147期号:3页码:460-465
文章类型Article
摘要The translationally controlled tumor protein (TCTP) is highly conserved and has been widely found in eukaryotic organisms. Here, we report the phylogenetic analysis and developmental expression of AmphiTCTP, a TCTP homologous gene in cephalochordate amphioxus. Phylogenetic analysis indicates that the putative protein of AmphiTCTP is close to its vertebrate orthologs. The mRNA of AmphiTCTP is found in fertilized eggs, early cleavage embryo and most of the early developmental stages by in situ hybridization and RT-PCR, but its expression is not detectable from late cleavage stage to mid-gastrula. The expression of AmphiTCTP in zygotes and early cleavage stages shows that AmphiTCTP may be a maternal gene. From the early neurula stage onward, AmphiTCTP transcript is localized in the presumptive notochord, presomitic mesoderm, and nascent somites. However, its expression is gradually down-regulated after the notochord and somites have been formed. The expression pattern of AmphiTCTP thus coincides with the differentiation of the notochord and somites, this suggests that AmphiTCTP may not be a housekeeping gene and may play an important role in mesoderm development. (c) 2007 Elsevier Inc. All rights reserved.; The translationally controlled tumor protein (TCTP) is highly conserved and has been widely found in eukaryotic organisms. Here, we report the phylogenetic analysis and developmental expression of AmphiTCTP, a TCTP homologous gene in cephalochordate amphioxus. Phylogenetic analysis indicates that the putative protein of AmphiTCTP is close to its vertebrate orthologs. The mRNA of AmphiTCTP is found in fertilized eggs, early cleavage embryo and most of the early developmental stages by in situ hybridization and RT-PCR, but its expression is not detectable from late cleavage stage to mid-gastrula. The expression of AmphiTCTP in zygotes and early cleavage stages shows that AmphiTCTP may be a maternal gene. From the early neurula stage onward, AmphiTCTP transcript is localized in the presumptive notochord, presomitic mesoderm, and nascent somites. However, its expression is gradually down-regulated after the notochord and somites have been formed. The expression pattern of AmphiTCTP thus coincides with the differentiation of the notochord and somites, this suggests that AmphiTCTP may not be a housekeeping gene and may play an important role in mesoderm development. (c) 2007 Elsevier Inc. All rights reserved.
关键词Amphioxus Development Notochord Somitogenesis Amphitctp
学科领域Biochemistry & Molecular Biology ; Zoology
DOI10.1016/j.cbpb.2007.02.012
URL查看原文
收录类别SCI
语种英语
WOS记录号WOS:000247555900013
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被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/5522
专题实验海洋生物学重点实验室
作者单位1.Shandong Univ, Life Sci Coll, Key Lab Expt Teratol Minist Educ, Jinan 250012, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
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Chen, Zhongke,Zhang, Hongwei,Yang, Hui,et al. The expression of AmphiTCTP, a TCTP orthologous gene in amphioxus related to the development of notochord and somites[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2007,147(3):460-465.
APA Chen, Zhongke,Zhang, Hongwei,Yang, Hui,Huang, Xiangwei,Zhang, Xiaohui,&Zhang, Peijun.(2007).The expression of AmphiTCTP, a TCTP orthologous gene in amphioxus related to the development of notochord and somites.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,147(3),460-465.
MLA Chen, Zhongke,et al."The expression of AmphiTCTP, a TCTP orthologous gene in amphioxus related to the development of notochord and somites".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 147.3(2007):460-465.
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