Isolation and characterization of a marine magnetotactic spirillum axenic culture QH-2 from an intertidal zone of the China Sea

doi:10.1016/j.resmic.2010.02.003

	Isolation and characterization of a marine magnetotactic spirillum axenic culture QH-2 from an intertidal zone of the China Sea
	Zhu, Kailing 2; Pan, Hongmiao 2; Li, Jinhua 3; Yu-Zhang, Kui 4; Zhang, Sheng-Da 2; Zhang, Wen-Yan 2; Zhou, Ke 2; Yue, Haidong 2; Pan, Yongxin 3,5; Xiao, Tian 2,5; Wu, Long-Fei 1,5
	2010-05-01
发表期刊	RESEARCH IN MICROBIOLOGY
ISSN	0923-2508
卷号	161 期号:4 页码:276-283
文章类型	Article
摘要	Magnetotactic bacteria (MTB) are ubiquitous in aquatic habitats. Because of their fastidious requirements for growth conditions, only very few axenic MTB cultures have been obtained worldwide. In this study, we report a novel marine magnetotactic spirillum axenic culture, designated as QH-2, isolated from the China Sea. It was able to grow in semi-solid or liquid chemically defined medium. The cells were amphitrichously flagellated and contained one single magnetosome chain with an average number of 16 magnetosomes per cell. Phosphate and lipid granules were also observed in the cells. Both rock magnetism and energy-dispersive X-ray spectroscopy characterizations indicated that the magnetosomes in QH-2 were single-domain magnetites (Fe(3)O(4)). QH-2 cells swam mostly in a straight line at a velocity of 20-50 mu m/s and occasionally changed to a helical motion. Unlike other magnetotactic spirilla. QH-2 cells responded to light illumination. As a consequence of illumination, the cells changed the direction in which they swam from parallel to the magnetic field to antiparallel. This response appears to be similar to the effect of an increase in [O(2)]. Analysis of the QH-2 16S rRNA sequence showed that it had greater than 11% sequence divergence from freshwater magnetotactic spirilla. Thus, the marine QH-2 strain seems to be both phylogenetically and magnetotactically distinct from the freshwater Magnetospirillum spp. studied previously. (C) 2010 Elsevier Masson SAS. All rights reserved.; Magnetotactic bacteria (MTB) are ubiquitous in aquatic habitats. Because of their fastidious requirements for growth conditions, only very few axenic MTB cultures have been obtained worldwide. In this study, we report a novel marine magnetotactic spirillum axenic culture, designated as QH-2, isolated from the China Sea. It was able to grow in semi-solid or liquid chemically defined medium. The cells were amphitrichously flagellated and contained one single magnetosome chain with an average number of 16 magnetosomes per cell. Phosphate and lipid granules were also observed in the cells. Both rock magnetism and energy-dispersive X-ray spectroscopy characterizations indicated that the magnetosomes in QH-2 were single-domain magnetites (Fe3O4). QH-2 cells swam mostly in a straight line at a velocity of 20-50 mu m/s and occasionally changed to a helical motion. Unlike other magnetotactic spirilla. QH-2 cells responded to light illumination. As a consequence of illumination, the cells changed the direction in which they swam from parallel to the magnetic field to antiparallel. This response appears to be similar to the effect of an increase in [O-2]. Analysis of the QH-2 16S rRNA sequence showed that it had greater than 11% sequence divergence from freshwater magnetotactic spirilla. Thus, the marine QH-2 strain seems to be both phylogenetically and magnetotactically distinct from the freshwater Magnetospirillum spp. studied previously. (C) 2010 Elsevier Masson SAS. All rights reserved.
关键词	Magnetotactic Bacteria Response To Light Pure Culture Magnetosome Phylogenetic Analysis
学科领域	Microbiology
DOI	10.1016/j.resmic.2010.02.003
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000279125600005
引用统计	被引频次：75[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5510
专题	海洋生态与环境科学重点实验室
作者单位	1.CNRS, Chim Bacterienne Lab, Inst Biol Struct & Microbiol, UPR9043, F-13009 Marseille, France 2.Chinese Acad Sci, Key Lab Marine Ecol & Environm Sci, Inst Oceanol, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Key Lab Earths Deep Interior, Inst Geol & Geophys, Beijing 100029, Peoples R China 4.Univ Reims, Lab Microscopies & Etud Nanostruct, F-51687 Reims, France 5.CNRS, Lab Int Associe Biomineralizat & NanoStruct, F-13009 Marseille, France
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhu, Kailing,Pan, Hongmiao,Li, Jinhua,et al. Isolation and characterization of a marine magnetotactic spirillum axenic culture QH-2 from an intertidal zone of the China Sea[J]. RESEARCH IN MICROBIOLOGY,2010,161(4):276-283.
APA	Zhu, Kailing.,Pan, Hongmiao.,Li, Jinhua.,Yu-Zhang, Kui.,Zhang, Sheng-Da.,...&Wu, Long-Fei.(2010).Isolation and characterization of a marine magnetotactic spirillum axenic culture QH-2 from an intertidal zone of the China Sea.RESEARCH IN MICROBIOLOGY,161(4),276-283.
MLA	Zhu, Kailing,et al."Isolation and characterization of a marine magnetotactic spirillum axenic culture QH-2 from an intertidal zone of the China Sea".RESEARCH IN MICROBIOLOGY 161.4(2010):276-283.

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