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Effect of alginic acid decomposing bacterium on the growth of Laminaria japonica (Phaeophyceae)
Wang, Y; Tang, XX; Yang, Z; Yu, ZM
2006
发表期刊JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA
ISSN1001-0742
卷号18期号:3页码:543-551
文章类型Article
摘要We collected the diseased blades of Laminaria japonica from Yantai Sea Farm from October to December 2002, and the alginic acid decomposing bacterium on the diseased blade was isolated and purified, and was identified as Alterornonas espejiana. This bacterium was applied as the causative pathogen to infect the blades of L. japonica under laboratory conditions. The aim of the present study was to identify the effects of the bacterium on the growth of L. japonica, and to find the possibly effective mechanism. Results showed that: (1) The blades of L. japonica exhibited symptoms of lesion, bleaching and deterioration when infected by the bacterium, and their growth and photosynthesis were dramatically suppressed. At the same time, the reactive oxygen species (ROS) generation enhanced obviously, and the relative membrane permeability increased significantly. The contents of malonaldehyde (MDA) and free fatty acid in the microsomol membrane greatly elevated, but the phospholipid content decreased. Result suggested an obvious peroxidation and deesterrification in the blades of L. japonica when infected by the bacterium. (2) The simultaneous assay on the antioxidant enzyme activities demonstrated that superoxide dismutase (SOD) and catalase (CAT) increased greatly when infected by the bacterium, but glutathione peroxidase (Gpx) and ascorbate peroxidase (APX) did not exhibit active responses to the bacterium throughout the experiment. (3) The histomorphological observations gave a distinctive evidence of the severity of the lesions as well as the relative abundance in the bacterial population on the blades after infection. The bacterium firstly invaded into the endodermis of L. japonica and gathered around there, and then resulted in the membrane damage, cells corruption and ultimately, the death of L. japonica.; We collected the diseased blades of Laminaria japonica from Yantai Sea Farm from October to December 2002, and the alginic acid decomposing bacterium on the diseased blade was isolated and purified, and was identified as Alterornonas espejiana. This bacterium was applied as the causative pathogen to infect the blades of L. japonica under laboratory conditions. The aim of the present study was to identify the effects of the bacterium on the growth of L. japonica, and to find the possibly effective mechanism. Results showed that: (1) The blades of L. japonica exhibited symptoms of lesion, bleaching and deterioration when infected by the bacterium, and their growth and photosynthesis were dramatically suppressed. At the same time, the reactive oxygen species (ROS) generation enhanced obviously, and the relative membrane permeability increased significantly. The contents of malonaldehyde (MDA) and free fatty acid in the microsomol membrane greatly elevated, but the phospholipid content decreased. Result suggested an obvious peroxidation and deesterrification in the blades of L. japonica when infected by the bacterium. (2) The simultaneous assay on the antioxidant enzyme activities demonstrated that superoxide dismutase (SOD) and catalase (CAT) increased greatly when infected by the bacterium, but glutathione peroxidase (Gpx) and ascorbate peroxidase (APX) did not exhibit active responses to the bacterium throughout the experiment. (3) The histomorphological observations gave a distinctive evidence of the severity of the lesions as well as the relative abundance in the bacterial population on the blades after infection. The bacterium firstly invaded into the endodermis of L. japonica and gathered around there, and then resulted in the membrane damage, cells corruption and ultimately, the death of L. japonica.
关键词Laminaria Japonica Alginic Acid Decomposing Bacterial Infection Growth Mechanism
学科领域Environmental Sciences
收录类别SCI
语种英语
WOS记录号WOS:000237568200022
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/5466
专题海洋生态与环境科学重点实验室
海洋环境腐蚀与生物污损重点实验室
作者单位1.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
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GB/T 7714
Wang, Y,Tang, XX,Yang, Z,et al. Effect of alginic acid decomposing bacterium on the growth of Laminaria japonica (Phaeophyceae)[J]. JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,2006,18(3):543-551.
APA Wang, Y,Tang, XX,Yang, Z,&Yu, ZM.(2006).Effect of alginic acid decomposing bacterium on the growth of Laminaria japonica (Phaeophyceae).JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,18(3),543-551.
MLA Wang, Y,et al."Effect of alginic acid decomposing bacterium on the growth of Laminaria japonica (Phaeophyceae)".JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA 18.3(2006):543-551.
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